Renaturation rate studies of a single family of interspersed repeated sequences in human deoxyribonucleic acid.
Renaturation rate studies of a single family of interspersed repeated sequences in human deoxyribonucleic acid.
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人脱氧核糖核酸中单个散布重复序列家族的复性率研究。
DOI：
10.1021/bi00514a003
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Schmid,CW
中科院分区：
文献类型：
--
作者：
Rinehart,FP;Ritch,TG;Deininger,PL;Schmid,CW
Frank P. Rinehart, Thomas G. Ritch, Prescott L. Deininger和Carl W. Schmid*摘要：我们研究了从人类DNA中分离的一个克隆的散布重复序列家族的恢复动力学。对单个克隆序列的交叉复性研究表明，由该序列家族成员形成的异源双链在复性速率和热稳定性方面存在异质性。然而，该家族的克隆成员在人类基因组中都具有大约相同的拷贝数，这表明根据DNA恢复动力学标准，它们是一个单一的序列家族。当该家族的单个克隆成员以总人类DNA作为温度的函数进行再生时，发现再生的异双链物的热稳定性与再生温度无关，在熔融温度低于25℃至高于4℃的范围内。此外，与该克隆家族成员发生反应的基因组拷贝数也与从25℃以下到融化温度的还原温度无关。这些观察结果表明，该家族成员的进化序列差异具有显著的同质性。这些结果也表明，恢复动力学可以准确地测量散布重复的基因组拷贝数
Frank P. Rinehart, Thomas G. Ritch, Prescott L. Deininger, and Carl W. Schmid* abstract: We have investigated the renaturation kinetics of a single family of cloned interspersed repeated sequences isolated from human DNA. Cross-renaturation studies of individual cloned sequences reveal heterogeneity in both the renaturation rateand thethermal stability of heteroduplexes formed from members of this family of sequences. However, cloned members of this family all renature with approximately the same number of copies in the human genome, demon-strating that they are a single family of sequences by the criterion of DNA renaturation kinetics. When a single cloned member of the family is renatured with total human DNA as a function of temperature, the thermal stability of the rena-tured heteroduplexes is found to be independent of the rena-turation temperature over the range 25 C below to 4 C above the melting temperature. Further, the number of genomic copies with which this cloned family member reacts is also independent of the renaturation temperature from 25 C below up to the melting temperatures. These observations demonstrate a remarkable degree of homogeneity in the evolutionary sequence divergence of members of this family. These results also demonstrate that renaturation kinetics can accurately measure the number of genomic copies of interspersed repeated
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影响因子：
64.8
作者：
Edwin M. Southern
通讯作者：
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DOI：
10.1073/pnas.77.3.1398
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
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作者：
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