Developmental exposure to lead causes inherent changes on voltage-gated sodium channels in rat hippocampal CA1 neurons
Developmental exposure to lead causes inherent changes on voltage-gated sodium channels in rat hippocampal CA1 neurons
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铅的发育暴露导致大鼠海马 CA1 神经元电压门控钠通道的固有变化
DOI：
10.1016/j.neuroscience.2008.02.016
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发表时间：
2008-05
期刊：
影响因子：
3.3
通讯作者：
Wang, Hui-li
中科院分区：
文献类型：
--
作者：
Liu, Ji;Chen, Ju-Tao;Ruan, Di -Yun;Tang, Jiu -Lai;Deng, Hong-Min;Ll, Chen-Chen;Ma, F. -L.;Wang, Lang.;Yan, Dan;Wang, Hui-li
在本研究中，使用传统的全细胞膜片钳技术在大鼠海马切片上研究了妊娠第 0 天 (E0) 至出生后第 15 天 (P15) 期间慢性铅 (Pb2+) 暴露对海马 (CA1) 神经元 CA1 区电压门控钠通道电流 (INa) 的影响。我们发现发育期铅暴露增加了激活阈值和诱发最大INa电流的电压，导致INa稳态激活曲线正移，并增大了INatail电流； Pb2+以电压依赖性方式延迟INa的激活，延长钠通道快速失活的时程； Pb2+引起稳态失活曲线右移，加速了INa的活性依赖性衰减，但对INa从失活恢复的时间过程和失活通道的分数没有显着影响。此外，与有效的抗氧化剂和自由基清除剂α-生育酚（VE）共同处理，完全阻止了上述INa的变化。发育期铅暴露引起的INa性质的改变与以往在更接近生理情况的条件下的急性实验有所不同，该过程被认为与铅参与脂质过氧化反应有关，有报道称该反应改变了膜蛋白的构象和生物物理功能。
In this study, the effects of chronic lead (Pb2+) exposure, during day 0 of gestation (E0) to postnatal day 15 (P15), on voltage-gated sodium channel currents (INa) were investigated in CA1 field of the hippocampus (CA1) neurons using the conventional whole-cell patch-clamp technique on rat hippocampal slices. We found that developmental lead exposure increased the activation threshold and the voltage at which the maximum INacurrent was evoked, caused positive shifts of INasteady-state activation curve, and enlarged INatail-currents; Pb2+delayed the activation of INain a voltage-dependent manner, prolonged the time course of the fast inactivation of sodium channels; Pb2+induced a right shift of the steady-state inactivation curve, accelerated the activity-dependent attenuation of INa, but made no significant effects on the time course of the recovery of INafrom inactivation and the fraction of inactivated channels. In addition, the co-treatment with α-tocopherol (VE), an effective antioxidant and free radical scavenger, completely prevented the aforementioned changes on INa. The alterations on INaproperties induced by developmental lead exposure were partly different from that in previous acute experiments under the conditions closer to physiological situation, and the process was considered related to the participating of lead in lipid peroxidation reaction, which has been reported to change the conformation and biophysical functions of membrane proteins.
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影响因子：
4.7
作者：
M. Mazzolini;S. Traverso;C. Marchetti
通讯作者：
M. Mazzolini;S. Traverso;C. Marchetti
影响因子：
3.8
作者：
J. Villeda‐Hernández;R. Barroso-Moguel;M. Méndez-Armenta;C. Nava-Ruíz;R. Huerta-Romero;Camilo Ríos
通讯作者：
J. Villeda‐Hernández;R. Barroso-Moguel;M. Méndez-Armenta;C. Nava-Ruíz;R. Huerta-Romero;Camilo Ríos
影响因子：
3.4
作者：
Sweatt, JD
通讯作者：
Sweatt, JD
影响因子：
2.5
作者：
Vijayaragavan, K;Boutjdir, M;Chahine, M
通讯作者：
Chahine, M
影响因子：
3.3
作者：
Kamal, A;Artola, A;Ramakers, GMJ
通讯作者：
Ramakers, GMJ