Multilayer microfluidic platform for the study of luminal, transmural, and interstitial flow.
Multilayer microfluidic platform for the study of luminal, transmural, and interstitial flow.
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用于研究腔内、跨壁和间隙流动的多层微流控平台。
DOI：
10.1088/1758-5090/ac48e5
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发表时间：
2022-01-25
期刊：
影响因子：
9
通讯作者：
Polacheck WJ
中科院分区：
文献类型：
--
作者：
Lee GH;Huang SA;Aw WY;Rathod ML;Cho C;Ligler FS;Polacheck WJ
有效地向组织输送氧气和营养物质需要血液、淋巴和间质液体压力的复杂平衡，而液体压力的梯度驱动血液、淋巴和间质液体在组织中的流动。虽然特定的流体机械刺激，如壁面切应力，已被证明调节细胞信号通路以及基因和蛋白质的表达模式，但由于现有分析的局限性，对流动流体传递的关键信号以及这些信号如何在天然组织中跨多种细胞和细胞类型整合的理解是不完整的。在这里，我们介绍了一种多层微流控平台(MμLTI-Flow)，该平台能够培养工程血液和淋巴微血管，并独立控制血液、淋巴管和间质流体的压力。使用光学显微镜方法测量施加输入压力的流体速度，我们演示了间质流体流动的不同速率作为血液、淋巴和间质压力的函数，这与计算流体动力学模型一致。由此产生的微流体和计算平台将提供对关键流体力学参数和细胞机制的分析，这些参数和细胞机制有助于导致流体失衡在疾病进展中发挥作用的疾病，包括淋巴水肿和实体癌症。
Efficient delivery of oxygen and nutrients to tissues requires an intricate balance of blood, lymphatic, and interstitial fluid pressures, and gradients in fluid pressure drive the flow of blood, lymph, and interstitial fluid through tissues. While specific fluid mechanical stimuli, such as wall shear stress, have been shown to modulate cellular signaling pathways along with gene and protein expression patterns, an understanding of the key signals imparted by flowing fluid and how these signals are integrated across multiple cells and cell types in native tissues is incomplete due to limitations with current assays. Here, we introduce a multi-layer microfluidic platform (MμLTI-Flow) that enables the culture of engineered blood and lymphatic microvessels and independent control of blood, lymphatic, and interstitial fluid pressures. Using optical microscopy methods to measure fluid velocity for applied input pressures, we demonstrate varying rates of interstitial fluid flow as a function of blood, lymphatic, and interstitial pressure, consistent with computational fluid dynamics models. The resulting microfluidic and computational platforms will provide for analysis of key fluid mechanical parameters and cellular mechanisms that contribute to diseases in which fluid imbalances play a role in progression, including lymphedema and solid cancer.
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影响因子：
64.8
作者：
Polacheck WJ;Kutys ML;Yang J;Eyckmans J;Wu Y;Vasavada H;Hirschi KK;Chen CS
通讯作者：
Chen CS
DOI：
10.1098/rsif.2017.0131
发表时间：
2017-05
期刊：
Journal of the Royal Society, Interface
影响因子：
--
作者：
Abbas Y;Oefner CM;Polacheck WJ;Gardner L;Farrell L;Sharkey A;Kamm R;Moffett A;Oyen ML
通讯作者：
Oyen ML
影响因子：
7.5
作者：
Kutys, Matthew L.;Chen, Christopher S.
通讯作者：
Chen, Christopher S.
影响因子：
2.7
作者：
FISHER, C;GILBERTSONBEADLING, S;MITCHELL, MA
通讯作者：
MITCHELL, MA
影响因子：
5.7
作者：
Doherty EL;Aw WY;Hickey AJ;Polacheck WJ
通讯作者：
Polacheck WJ