Microfluidic and Organ-on-a-Chip Approaches to Investigate Cellular and Microenvironmental Contributions to Cardiovascular Function and Pathology.
Microfluidic and Organ-on-a-Chip Approaches to Investigate Cellular and Microenvironmental Contributions to Cardiovascular Function and Pathology.
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DOI：
10.3389/fbioe.2021.624435
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发表时间：
2021
影响因子：
5.7
通讯作者：
Polacheck WJ
中科院分区：
文献类型：
--
作者：
Doherty EL;Aw WY;Hickey AJ;Polacheck WJ
在过去的十年中，微加工和生物材料的进步促进了微流体组织和器官模型的发展，以应对传统动物和细胞培养系统的挑战。这些系统主要用于人类疾病建模和临床前药物开发，并越来越多地用于了解细胞和分子机制，特别是在传统系统中难以概括其特征力学和结构的心血管系统中。在这里，我们回顾了最近的微流体方法来模拟心血管系统和这些系统提供的新见解。微流体方法的主要特点包括在细胞长度尺度上对细胞和细胞外基质（ECM）进行模式化的能力，以及使用患者来源细胞的能力。我们将重点回顾利用这些特征来探索心血管疾病进展中基因突变与微环境之间关系的方法。此外，我们讨论了各种方法的局限性和优点，并考虑了微加工技术和生物化学技术的进一步发展在建立微流控心血管疾病模型方面的作用，作为理解生物机制和制定介入策略的核心工具。
Over the past decade, advances in microfabrication and biomaterials have facilitated the development of microfluidic tissue and organ models to address challenges with conventional animal and cell culture systems. These systems have largely been developed for human disease modeling and preclinical drug development and have been increasingly used to understand cellular and molecular mechanisms, particularly in the cardiovascular system where the characteristic mechanics and architecture are difficult to recapitulate in traditional systems. Here, we review recent microfluidic approaches to model the cardiovascular system and novel insights provided by these systems. Key features of microfluidic approaches include the ability to pattern cells and extracellular matrix (ECM) at cellular length scales and the ability to use patient-derived cells. We focus the review on approaches that have leveraged these features to explore the relationship between genetic mutations and the microenvironment in cardiovascular disease progression. Additionally, we discuss limitations and benefits of the various approaches, and conclude by considering the role further advances in microfabrication technology and biochemistry techniques play in establishing microfluidic cardiovascular disease models as central tools for understanding biological mechanisms and for developing interventional strategies.
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影响因子：
4
作者：
Daley, William P.;Yamada, Kenneth M.
通讯作者：
Yamada, Kenneth M.
影响因子：
4.4
作者：
BARTH, PG;SCHOLTE, HR;SOBOTKAPLOJHAR, MA
通讯作者：
SOBOTKAPLOJHAR, MA
影响因子：
4.3
作者：
Cox TR;Erler JT
通讯作者：
Erler JT
影响因子：
4.1
作者：
Boudou, Thomas;Legant, Wesley R.;Chen, Christopher S.
通讯作者：
Chen, Christopher S.
DOI：
10.1046/j.1443-9506.2003.t01-1-.x
发表时间：
2003-01-01
期刊：
Heart, lung & circulation
影响因子：
--
作者：
Field, Peter L
通讯作者：
Field, Peter L