A jacalin-related lectin-like gene in wheat is a component of the plant defence system.
A jacalin-related lectin-like gene in wheat is a component of the plant defence system.
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小麦中与jacalin相关的凝集素样基因是植物防御系统的一个组成部分
DOI：
10.1093/jxb/err226
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发表时间：
2011-11
影响因子：
6.9
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Xiang Y;Song M;Wei Z;Tong J;Zhang L;Xiao L;Ma Z;Wang Y
Jacalin-related lectins（JRL）是具有一个或多个jacalin样凝集素结构域的蛋白质亚组。尽管JRL通常与生物或非生物刺激有关，但其在植物中的生物学功能以及与植物抗病性的关系知之甚少。在小麦中发现了一个甘露糖特异性JRL（mJRL）样基因（TaJRLL 1），该基因主要在茎和穗中表达，编码一个具有两个jacalin样凝集素结构域的蛋白。病原体感染和植物激素处理诱导其表达，而水杨酸（SA）的生物合成抑制剂多效唑和茉莉酸（JA）的生物合成抑制剂二乙基二硫代氨基甲酸的应用，分别大幅抑制其表达。通过病毒诱导的基因沉默减弱TaJRLL 1增加了对兼性真菌病原体禾谷镰刀菌和活体营养型真菌病原体小麦白粉病菌的易感性。转化TaJRLL 1的拟南芥对F. graminearum和灰葡萄孢Botrytis cinerea。转基因拟南芥中JA和SA水平显著增加。由于TaJRLL 1功能的改变而导致的抗病性的丧失或增加与SA和JA依赖性防御信号通路的减弱或增强相关。这些结果表明，TaJRLL 1可能是SA和JA依赖的防御信号通路的一个组成部分。
Jacalin-related lectins (JRLs) are a subgroup of proteins with one or more jacalin-like lectin domains. Although JRLs are often associated with biotic or abiotic stimuli, their biological functions in plants, as well as their relationships to plant disease resistance, are poorly understood. A mannose-specific JRL (mJRL)-like gene (TaJRLL1) that is mainly expressed in stem and spike and encodes a protein with two jacalin-like lectin domains was identified in wheat. Pathogen infection and phytohormone treatments induced its expression; while application of the salicylic acid (SA) biosynthesis inhibitor paclobutrazol and the jasmonic acid (JA) biosynthesis inhibitor diethyldithiocarbamic acid, respectively, substantially inhibited its expression. Attenuating TaJRLL1 through virus-induced gene silencing increased susceptibility to the facultative fungal pathogen Fusarium graminearum and the biotrophic fungal pathogen Blumeria graminis. Arabidopsis thaliana transformed with TaJRLL1 displayed increased resistance to F. graminearum and Botrytis cinerea. JA and SA levels in transgenic Arabidopsis increased significantly. A loss or increase of disease resistance due to an alteration in TaJRLL1 function was correlated with attenuation or enhancement of the SA- and JA-dependent defence signalling pathways. These results suggest that TaJRLL1 could be a component of the SA- and JA-dependent defence signalling pathways.
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