Cross-presentation of antigen by diverse subsets of murine liver cells.
Cross-presentation of antigen by diverse subsets of murine liver cells.
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DOI：
10.1002/hep.24508
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发表时间：
2011-10
期刊：
影响因子：
13.5
通讯作者：
Crispe, Ian N.
中科院分区：
文献类型：
--
作者：
Ebrahimkhani, Mohammad R.;Mohar, Isaac;Crispe, Ian N.
抗原交叉呈递是骨髓来源的专门抗原呈递细胞（例如树突状细胞）的主要功能。虽然这些细胞有时被称为“专业”抗原呈递细胞，但非骨髓来源的细胞也可以充当抗原呈递细胞。在这里，通过四路肝细胞分离和候选抗原呈递细胞的平行比较，我们表明，根据抗原供体细胞的丰度，不同的肝细胞亚群可以交叉呈递肝细胞相关抗原。即使在非常低的抗原浓度以及使用可溶性蛋白时，在肝窦内皮细胞和库普弗细胞中也观察到这种功能。肝细胞的抗原交叉呈递诱导有效的 CD8+ T 细胞增殖，其方式与来自脾脏的经典树突状细胞类似。然而，增殖细胞表达较低水平的 T 细胞激活标记物和细胞内干扰素-γ 水平。与经典的脾树突状细胞相反，肝脏抗原呈递细胞的交叉呈递主要依赖于细胞间粘附分子-1。肝窦是一个富含抗原交叉呈递活性的环境。然而，肝脏中常驻的抗原呈递细胞会导致部分 T 细胞激活。这些结果阐明了肝脏如何作为 CD8+ T 细胞激活的主要部位，以及为什么针对肝细胞病原体的免疫有时无效
Antigen cross-presentation is a principal function of specialized antigen-presenting cells of bone marrow origin such as dendritic cells. While these cells are sometimes known as “professional” antigen-presenting cells, non-bone marrow derived cells may also act as antigen-presenting cells. Here, using four-way liver cell isolation and parallel comparison of candidate antigen presenting cells, we show that depending on the abundance of antigen-donor cells, different subsets of liver cells could cross-present a hepatocyte-associated antigen. This function was observed in both liver sinusoidal endothelial cells and Kupffer cells even at very low antigen concentration, as well as when using soluble protein. Antigen cross-presentation by liver cells induced efficient CD8+ T cell proliferation in a similar manner to classical dendritic cells from spleen. However, proliferated cells expressed lower level of T-cell activation markers and intracellular interferon-gamma levels. In contrast to classical spleen dendritic cells, cross-presentation by liver antigen presenting cells was predominantly dependent on Intercellular Adhesion Molecule-1. Hepatic sinusoids are an environment rich in antigen cross-presenting activity. However the liver's resident antigen-presenting cells cause partial T-cell activation. These results clarify how the liver can act as a primary site of CD8+ T-cell activation, and why immunity against hepatocyte pathogens is sometimes ineffective
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影响因子：
13.5
作者：
Tuyama, Ana C.;Hong, Feng;Saiman, Yedidya;Wang, Chuansheng;Ozkok, Derya;Mosoian, Arevik;Chen, Ping;Chen, Benjamin K.;Klotman, Mary E.;Bansal, Meena B.
通讯作者：
Bansal, Meena B.
影响因子：
4.4
作者：
Burgdorf, Sven;Lukacs-Kornek, Veronika;Kurts, Christian
通讯作者：
Kurts, Christian
影响因子：
32.4
作者：
Winau, Florian;Hegasy, Guido;Kaufmann, Stefan H. E.
通讯作者：
Kaufmann, Stefan H. E.
影响因子：
32.4
作者：
Bird, JJ;Brown, DR;Reiner, SL
通讯作者：
Reiner, SL
DOI：
10.1084/jem.194.10.1395
发表时间：
2001-11-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Thimme R;Oldach D;Chang KM;Steiger C;Ray SC;Chisari FV
通讯作者：
Chisari FV