T lymphocyte colony assay in hemophiliacs
T lymphocyte colony assay in hemophiliacs
复制标题
血友病患者 T 淋巴细胞集落测定
DOI：
--
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
B. Rabin
中科院分区：
文献类型：
--
作者：
M. Ragni;A. Winkelstein;T. Evans;J. Lewis;F. Bontempo;J. Spero;B. Rabin
不明原因的淋巴结病，有或没有伴随的症状，被称为“淋巴结病综合征”，已被确认在获得性免疫缺陷综合征（艾滋病）的风险群体，即同性恋者和血友病患者。然而，到目前为止，还没有确定的测试，区分无症状的个人和那些在这些高危人群中的腺病。在无症状血友病患者中评价了T集落试验，该试验在软琼脂中测量T淋巴细胞生长，并允许选择性T细胞增殖，细胞间接触最少。8名血友病患者淋巴结肿大（HA）的平均菌落数（763 +/- 348（+/- SEM））显著低于16名健康血友病患者（HH）的平均菌落数（3，044 +/- 661（P <0.005）），或低于24名异性恋对照受试者的平均菌落数（3，964 +/- 395（P <0.005）。在体外添加外源性白细胞介素-2（IL- 2）恢复了HA群体的正常集落生长。这些结果表明，T细胞集落试验可以检测异常的细胞介导的免疫血友病患者和特异性区分无症状血友病（HH）和腺病（HA）。此外，IL-2可能在改善艾滋病或“淋巴结病综合征”中的T细胞缺陷方面具有潜在益处;然而，这仍有待证实。
Unexplained lymphadenopathy, with or without accompanying symptoms, known as the “lymphadenopathy syndrome,” has been recognized in groups at risk for acquired immune deficiency syndrome (AIDS), namely, homosexuals and hemophiliacs. To date, however, no test has been defined that discriminates between asymptomatic individuals and those with adenopathy in these high-risk groups. The T colony assay, which measures T lymphocyte growth in soft agar and which allows selective T cell proliferation with minimal cell-cell contact, was evaluated in asymptomatic hemophiliacs. Significantly lower mean colony counts were found in eight hemophiliacs with adenopathy (HA), 763 +/- 348 (+/- SEM), than in 16 healthy hemophiliacs (HH) 3,044 +/- 661 (P less than .005), or than in 24 heterosexual control subjects, 3,964 +/- 395 (P less than .005). The in vitro addition of exogenous interleukin-2 (IL- 2) restored normal colony growth in the HA population. These results indicate that the T colony assay can detect abnormal cell-mediated immunity among hemophiliacs and specifically discriminates between asymptomatic hemophiliacs (HH) and those with adenopathy (HA). In addition, IL-2 may be of potential benefit in improving T cell defects in AIDS or the “lymphadenopathy syndrome”; however, this remains to be proven.
DOI：
10.1016/0002-9343(82)90174-7
发表时间：
1982
期刊：
The American journal of medicine
影响因子：
--
作者：
Stahl,RE;Friedman-Kien,A;Dubin,R;Marmor,M;Zolla-Pazner,S
通讯作者：
Zolla-Pazner,S
用冷沉淀和冻干浓缩物治疗的经典血友病患者的 T 淋巴细胞亚群。
DOI：
10.1056/nejm198301133080206
发表时间：
1983
期刊：
The New England journal of medicine
影响因子：
--
作者：
Menitove,JE;Aster,RH;Casper,JT;Lauer,SJ;Gottschall,JL;Williams,JE;Gill,JC;Wheeler,DV;Piaskowski,V;Kirchner,P;Montgomery,RR
通讯作者：
Montgomery,RR
人 T 淋巴细胞集落形成对辅助细胞或肿瘤细胞产生的可溶性因子的依赖性。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Winkelstein,A
通讯作者：
Winkelstein,A