Transforming growth factor beta-1 in acute myocardial infarction in rats.
Transforming growth factor beta-1 in acute myocardial infarction in rats.
复制标题
转化生长因子β-1在大鼠急性心肌梗死中的作用。
作者：
N. L. Thompson;F. Bazoberry;Edith Speir;W. Casscells;V. Ferrans;K. Flanders;Patljru Kondaiah;Andrew G. Geiser;M. B. Sporn
在左冠状动脉结扎引起的心肌梗死期间，对心脏中的TGF-β 1进行了检查。通过TGF-β 1的免疫组织化学染色和mRNA的北方印迹分析比较了梗死和未梗死心肌。正常心室肌细胞被TGF-β 1抗体强烈染色。冠状动脉结扎后1小时内，这些肌细胞的染色开始逐渐丧失。然而，在结扎后24-48小时，在梗塞区域的边缘处观察到肌细胞的强烈染色。结扎后48小时，梗死心肌的北方印迹显示主要的2.4 kb TGF-β 1 mRNA增加了3- 4倍;次要的1.9 kb转录物也显著增加。在相同的组织样本中，糖酵解酶甘油醛-3-磷酸脱氢酶的mRNA减少了2倍。结果表明，TGF-β在心脏对损伤的反应中起着重要作用。
TGF-beta 1 has been examined in the heart during myocardial infarction caused by ligation of the left coronary artery. Infarcted and uninfarcted myocardium have been compared by immunohistochemical staining of TGF-beta 1 and by Northern blot analysis of mRNA. Normal ventricular myocytes are strongly stained by an antibody to TGF-beta 1. Progressive loss of staining of these myocytes begins within 1 hr after coronary ligation. However, by 24-48 hr after ligation, intense staining of myocytes at the margin of infarcted areas is seen. Northern blots of infarcted myocardium 48 hr after ligation show a 3- to 4-fold increase in the principal 2.4 kb TGF-beta 1 mRNA; there is also a marked increase in a minor 1.9 kb transcript. In the same tissue samples, there is a 2-fold decrease in the mRNA for the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase. The results indicate a significant role for TGF-beta in the response of the heart to injury.
DOI：
10.1073/pnas.84.17.6020
发表时间：
1987-09-01
影响因子：
11.1
作者：
ASSOIAN, RK;FLEURDELYS, BE;SPORN, MB
通讯作者：
SPORN, MB