Phage display in molecular imaging and diagnosis of cancer.
Phage display in molecular imaging and diagnosis of cancer.
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DOI：
10.1021/cr900317f
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发表时间：
2010-05-12
期刊：
影响因子：
62.1
通讯作者：
Deutscher, Susan L.
中科院分区：
文献类型：
--
作者：
Deutscher, Susan L.
新的肽为基础的探针，以促进疾病的分子成像正在迅速发展，由于实施组合化学和噬菌体（噬菌体）展示。噬菌体展示是一种强大的技术，其允许大的序列空间筛选，提供了一种提高肽亲和力并产生结合任何给定靶标的独特肽的手段。自1985年问世以来，已经使用噬菌体展示分离和研究了数千种肽。这些肽正在探索在疫苗开发，酶抑制，炎症，植物病理，心血管疾病，癌症等。这篇综述的目的是分析和描述那些从噬菌体展示已成功地使用在过去五年中，在无线电和光学在体内肿瘤成像获得的肽。新的肿瘤靶向剂需要推进癌症的诊断和治疗，噬菌体展示选择的肽可能是一个有吸引力的手段来获得这样的代理。不幸的是，针对肿瘤抗原选择的数百种肽中的绝大多数尚未显示出在体内作为癌症分子成像剂的功能。近年来，在将多肽从体外翻译到体内应用方面取得了进展。噬菌体上展示的肽不仅在体内用作肿瘤成像剂，而且噬菌体本身已用于与许多标记平台的成像。噬菌体不仅作为发现肽的载体，而且作为纳米材料的整合具有广泛的应用。噬菌体展示技术作为一种强大、经济、快速和有效的方法正在发展用于癌症分子成像和诊断的新药物。
New peptide-based probes to facilitate the molecular imaging of disease are rapidly evolving due to implementation of combinatorial chemistry and bacteriophage (phage) display. Phage display is a powerful technique that allows vast sequence space screening, providing a means to improve peptide affinity and generate unique peptides that bind any given target. Since its inception in 1985, many thousands of peptides have been isolated and investigated using phage display. Such peptides are being explored in vaccine development, enzyme inhibition, inflammation, plant pathology, cardiovascular disease, cancer, etc. The purpose of this review is to analyze and describe those peptides obtained from phage display that have been used successfully in the past five years in both radio and optical in vivo tumor imaging. New tumor targeting agents are required to advance cancer diagnosis and treatment, and phage display selected peptides may be an attractive means to obtain such agents. Unfortunately, the vast majority of the hundreds of peptides selected against tumor antigens have not been shown to function as cancer molecular imaging agents in vivo. Recently, progress has been made in translation of the peptides from in vitro to in vivo applications. Not only have the peptides displayed on phage been employed in vivo as tumor imaging agents, but the phage themselves have been used in imaging with a number of labeling platforms. The integration of phage as not only vehicles for peptide discovery but also as a nanomaterial has wide-ranging applications. Phage display technology is emerging as a powerful, economical, rapid, and efficacious approach to develop new agents for the molecular imaging and diagnosis of cancer.
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DOI：
10.1073/pnas.152463399
发表时间：
2002-10-01
影响因子：
11.1
作者：
Åkerman, ME;Chan, WCW;Ruoslahti, E
通讯作者：
Ruoslahti, E
DOI：
10.1073/pnas.241655998
发表时间：
2002-02-05
影响因子：
11.1
作者：
Arap, W;Haedicke, W;Ruoslahti, E
通讯作者：
Ruoslahti, E
影响因子：
3.5
作者：
Böckmann, M;Drosten, M;Pützer, BM
通讯作者：
Pützer, BM
影响因子：
3.7
作者：
Chang DK;Lin CT;Wu CH;Wu HC
通讯作者：
Wu HC
影响因子：
82.9
作者：
Arap, W;Kolonin, MG;Pasqualini, R
通讯作者：
Pasqualini, R