Distinct patterns of histone acetyltransferase and Mediator deployment at yeast protein-coding genes.
Distinct patterns of histone acetyltransferase and Mediator deployment at yeast protein-coding genes.
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DOI：
10.1101/gad.312173.118
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发表时间：
2018-09-01
影响因子：
10.5
通讯作者：
Shore D
中科院分区：
文献类型：
--
作者：
Bruzzone MJ;Grünberg S;Kubik S;Zentner GE;Shore D
在这里，布鲁佐内等人。通过快速核消耗关键复合物亚基，探索转录共激活因子 Mediator 和两个组蛋白乙酰转移酶 (HAT) 复合物 NuA4 和 SAGA 对特定基因和基因类别的 RNA 聚合酶 II 关联的相对贡献。他们表明，NuA4 HAT Esa1 对某些基因组有不同的影响，而 SAGA HAT Gcn5 的影响较弱但更均匀，他们的发现表明至少三种不同的共激活剂部署组合可用于产生中等或较高的转录水平。转录共激活因子 Mediator 和两个组蛋白乙酰转移酶 (HAT) 复合物 NuA4 和 SAGA 在转录激活中发挥全局作用。在这里，我们通过快速核消耗关键复杂亚基来探索这些因素对特定基因和基因类别的 RNA 聚合酶 II 关联的相对贡献。我们发现 NuA4 HAT Esa1 对某些基因组有不同的影响，而 SAGA HAT Gcn5 的影响较弱但更均匀。对 Esa1 和 Tra1（NuA4 和 SAGA 的共同组成部分）的相对依赖性区分了两大类共同调控的生长促进基因。相比之下，我们表明介体的活性对于一小部分高度转录的含有 TATA-box 的基因尤其重要。我们的分析表明，至少三种不同的共激活剂部署组合用于产生中等或高转录水平，并表明每种组合可能与不同形式的调节相关。
Here, Bruzzone et al. explore the relative contributions of the transcriptional coactivators Mediator and two histone acetyltransferase (HAT) complexes, NuA4 and SAGA, to RNA polymerase II association at specific genes and gene classes by rapid nuclear depletion of key complex subunits. They show that the NuA4 HAT Esa1 differentially affects certain groups of genes, whereas the SAGA HAT Gcn5 has a weaker but more uniform effect, and their findings suggest that at least three distinct combinations of coactivator deployment are used to generate moderate or high transcription levels. The transcriptional coactivators Mediator and two histone acetyltransferase (HAT) complexes, NuA4 and SAGA, play global roles in transcriptional activation. Here we explore the relative contributions of these factors to RNA polymerase II association at specific genes and gene classes by rapid nuclear depletion of key complex subunits. We show that the NuA4 HAT Esa1 differentially affects certain groups of genes, whereas the SAGA HAT Gcn5 has a weaker but more uniform effect. Relative dependence on Esa1 and Tra1, a shared component of NuA4 and SAGA, distinguishes two large groups of coregulated growth-promoting genes. In contrast, we show that the activity of Mediator is particularly important at a separate, small set of highly transcribed TATA-box-containing genes. Our analysis indicates that at least three distinct combinations of coactivator deployment are used to generate moderate or high transcription levels and suggests that each may be associated with distinct forms of regulation.
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