Whole Genome Expression Profiling and Signal Pathway Screening of MSCs in Ankylosing Spondylitis.
Whole Genome Expression Profiling and Signal Pathway Screening of MSCs in Ankylosing Spondylitis.
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强直性脊柱炎间充质干细胞的全基因组表达谱和信号通路筛选
DOI：
10.1155/2014/913050
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发表时间：
2014
影响因子：
4.3
通讯作者：
Shen H
中科院分区：
文献类型：
--
作者：
Li Y;Wang P;Xie Z;Huang L;Yang R;Gao L;Tang Y;Zhang X;Ye J;Chen K;Cai Z;Wu Y;Shen H
强直性脊柱炎（AS）中间充质干细胞（MSCs）免疫调节功能失调的发病机制被认为是一个涉及多种遗传改变的复杂过程。在这项研究中，来自健康供体和AS患者的MSCs在正常培养基或模拟炎症环境的培养基中培养。对33,351个基因进行了全基因组表达谱分析，并通过GO术语分析和KEGG通路分析分析了与AS相关的差异表达基因。我们的研究结果显示，在正常培养基中，676个基因在AS中差异表达，354个基因上调，322个基因下调，而在炎症环境中，1767个基因在AS中差异表达，1230个基因上调，537个基因下调。GO分析表明，这些基因主要与细胞过程、生理过程、生物调控、生物过程调控、结合等相关。此外，通过KEGG通路分析，鉴定出14个MAPK信号通路关键基因和8个TLR信号通路关键基因存在差异调控。qRT-PCR结果验证了上述9个基因的表达变异。我们的研究发现，在炎症环境下强直性脊柱炎的发病机制可能与MAPK和TLR信号通路的激活有关。
The pathogenesis of dysfunctional immunoregulation of mesenchymal stem cells (MSCs) in ankylosing spondylitis (AS) is thought to be a complex process that involves multiple genetic alterations. In this study, MSCs derived from both healthy donors and AS patients were cultured in normal media or media mimicking an inflammatory environment. Whole genome expression profiling analysis of 33,351 genes was performed and differentially expressed genes related to AS were analyzed by GO term analysis and KEGG pathway analysis. Our results showed that in normal media 676 genes were differentially expressed in AS, 354 upregulated and 322 downregulated, while in an inflammatory environment 1767 genes were differentially expressed in AS, 1230 upregulated and 537 downregulated. GO analysis showed that these genes were mainly related to cellular processes, physiological processes, biological regulation, regulation of biological processes, and binding. In addition, by KEGG pathway analysis, 14 key genes from the MAPK signaling and 8 key genes from the TLR signaling pathway were identified as differentially regulated. The results of qRT-PCR verified the expression variation of the 9 genes mentioned above. Our study found that in an inflammatory environment ankylosing spondylitis pathogenesis may be related to activation of the MAPK and TLR signaling pathways.
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影响因子：
15.3
作者：
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