Genetic Characterisation of Plasmodium falciparum Isolates with Deletion of the pfhrp2 and/or pfhrp3 Genes in Colombia: The Amazon Region, a Challenge for Malaria Diagnosis and Control.
Genetic Characterisation of Plasmodium falciparum Isolates with Deletion of the pfhrp2 and/or pfhrp3 Genes in Colombia: The Amazon Region, a Challenge for Malaria Diagnosis and Control.
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DOI：
10.1371/journal.pone.0163137
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Murillo Solano C
中科院分区：
文献类型：
--
作者：
Dorado EJ;Okoth SA;Montenegro LM;Diaz G;Barnwell JW;Udhayakumar V;Murillo Solano C
大多数恶性疟原虫检测快速诊断试验（RDTs）的目标是富组氨酸蛋白2 （PfHRP2）。然而，已经检测到pfhrp2基因及其同源基因pfhrp3缺失的恶性疟原虫分离株。我们对2003年至2012年期间从哥伦比亚7个恶性疟原虫流行点采集的365份恶性疟原虫干血样本进行了广泛调查，以确定该国pfhrp2和/或pfhrp3阴性的恶性疟原虫的遗传特征和地理图谱。我们发现，只有亚马逊地区pfhrp2阴性的寄生虫比例很高（15/39;38.5%），这些寄生虫也是pfhrp3阴性的。这些寄生虫于2008年至2009年在亚马逊地区收集，而pfhrp3阴性寄生虫（157/365,43%）在所有地点和评估的每个样本收集年份（2003年至2012年）均被发现。我们还发现所有pfhrp2-和/或pfhrp3-阴性的寄生虫也对一个或两个侧翼基因呈阴性。共建立6个亚种群聚类，93.3%（14/15）的pfhrp2阴性寄生虫属于同一聚类，具有相同的单倍型。该单倍型与遗传谱系BV1相一致，BV1是一种多药耐药菌株，在2010年至2013年期间在秘鲁报告了两次暴发。早在2006年，我们就在哥伦比亚亚马逊地区发现了BV1的血统。在哥伦比亚的这些寄生虫中鉴定出两个新的克隆谱系：遗传谱系EV1和F. PfHRP2序列分析显示，在氨基酸水平上具有较高的遗传多样性，在分析的53个PfHRP2序列中鉴定出17个独特的序列。亚马逊地区存在pfhrp2缺失的疟原虫比例较高（38.5%），因此不推荐使用基于pfhrp2的RDTs，必须优先考虑在亚马逊地区实施新的疟疾诊断和控制策略。此外，考虑到美洲地区发生的广泛人类迁徙，有必要对该地区fpfhrp2阴性恶性疟原虫进行监测和遗传特征研究。
Most Plasmodium falciparum-detecting rapid diagnostic tests (RDTs) target histidine-rich protein 2 (PfHRP2). However, P. falciparum isolates with deletion of the pfhrp2 gene and its homolog gene, pfhrp3, have been detected. We carried out an extensive investigation on 365 P. falciparum dried blood samples collected from seven P. falciparum endemic sites in Colombia between 2003 and 2012 to genetically characterise and geographically map pfhrp2- and/or pfhrp3-negative P. falciparum parasites in the country. We found a high proportion of pfhrp2-negative parasites only in Amazonas (15/39; 38.5%), and these parasites were also pfhrp3-negative. These parasites were collected between 2008 and 2009 in Amazonas, while pfhrp3-negative parasites (157/365, 43%) were found in all the sites and from each of the sample collection years evaluated (2003 to 2012). We also found that all pfhrp2- and/or pfhrp3-negative parasites were also negative for one or both flanking genes. Six sub-population clusters were established with 93.3% (14/15) of the pfhrp2-negative parasites grouped in the same cluster and sharing the same haplotype. This haplotype corresponded with the genetic lineage BV1, a multidrug resistant strain that caused two outbreaks reported in Peru between 2010 and 2013. We found this BV1 lineage in the Colombian Amazon as early as 2006. Two new clonal lineages were identified in these parasites from Colombia: the genetic lineages EV1 and F. PfHRP2 sequence analysis revealed high genetic diversity at the amino acid level, with 17 unique sequences identified among 53 PfHRP2 sequences analysed. The use of PfHRP2-based RDTs is not recommended in Amazonas because of the high proportion of parasites with pfhrp2 deletion (38.5%), and implementation of new strategies for malaria diagnosis and control in Amazonas must be prioritised. Moreover, studies to monitor and genetically characterise pfhrp2-negative P. falciparum parasites in the Americas are warranted, given the extensive human migration occurring in the region.
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DOI：
10.1093/infdis/jit183
发表时间：
2013-08-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Polley SD;González IJ;Mohamed D;Daly R;Bowers K;Watson J;Mewse E;Armstrong M;Gray C;Perkins MD;Bell D;Kanda H;Tomita N;Kubota Y;Mori Y;Chiodini PL;Sutherland CJ
通讯作者：
Sutherland CJ
影响因子：
3.7
作者：
Akinyi Okoth S;Abdallah JF;Ceron N;Adhin MR;Chandrabose J;Krishnalall K;Huber CS;Goldman IF;Macedo de Oliveira A;Barnwell JW;Udhayakumar V
通讯作者：
Udhayakumar V
影响因子：
3.7
作者：
Gamboa D;Ho MF;Bendezu J;Torres K;Chiodini PL;Barnwell JW;Incardona S;Perkins M;Bell D;McCarthy J;Cheng Q
通讯作者：
Cheng Q
影响因子：
3.7
作者：
Murillo Solano C;Akinyi Okoth S;Abdallah JF;Pava Z;Dorado E;Incardona S;Huber CS;Macedo de Oliveira A;Bell D;Udhayakumar V;Barnwell JW
通讯作者：
Barnwell JW
影响因子：
9.4
作者：
Houze, Sandrine;Hubert, Veronique;Clain, Jerome
通讯作者：
Clain, Jerome