Metagenomic data-mining reveals contrasting microbial populations responsible for trimethylamine formation in human gut and marine ecosystems.
Metagenomic data-mining reveals contrasting microbial populations responsible for trimethylamine formation in human gut and marine ecosystems.
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DOI：
10.1099/mgen.0.000080
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发表时间：
2016-09
影响因子：
3.9
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Jameson E;Doxey AC;Airs R;Purdy KJ;Murrell JC;Chen Y
来自许多不同环境的现有宏基因组数据集包含了解代谢途径及其生物影响的未开发潜力。我们的兴趣在于三甲胺（TMA）的形成，这是人类健康和气候变化的关键代谢物。在这里，我们专注于胆碱，肉毒碱，甘氨酸甜菜碱和三甲胺N-氧化物（TMAO）的TMA在人类肠道和海洋宏基因组的细菌降解途径。我们发现TMAO还原酶途径是两种环境中最普遍的途径。变形菌被发现贡献的TMAO还原酶途径序列的大部分，除了在强调肠道，其中放线菌占主导地位。有趣的是，在人类肠道宏基因组中，克雷伯氏菌属和埃希氏菌属占变形菌门命中的高比例。此外，克雷伯氏菌和埃希氏菌具有四种潜在的TMA生产途径中的三种（胆碱，肉毒碱和TMAO），这表明它们在人类肠道中的TMA循环中具有关键作用。除了密集的TMAO-TMA在海洋环境中的循环，我们的数据表明，肉毒碱到TMA的转化在有氧海洋表面沃茨中起着被忽视的作用，而胆碱到TMA的转化是重要的厌氧海洋沉积物。我们的研究为在两种截然不同的环境中形成TMA的潜在关键微生物和代谢途径提供了新的见解。
Existing metagenome datasets from many different environments contain untapped potential for understanding metabolic pathways and their biological impact. Our interest lies in the formation of trimethylamine (TMA), a key metabolite in both human health and climate change. Here, we focus on bacterial degradation pathways for choline, carnitine, glycine betaine and trimethylamine N-oxide (TMAO) to TMA in human gut and marine metagenomes. We found the TMAO reductase pathway was the most prevalent pathway in both environments. Proteobacteria were found to contribute the majority of the TMAO reductase pathway sequences, except in the stressed gut, where Actinobacteria dominated. Interestingly, in the human gut metagenomes, a high proportion of the Proteobacteria hits were accounted for by the genera Klebsiella and Escherichia. Furthermore Klebsiella and Escherichia harboured three of the four potential TMA-production pathways (choline, carnitine and TMAO), suggesting they have a key role in TMA cycling in the human gut. In addition to the intensive TMAO–TMA cycling in the marine environment, our data suggest that carnitine-to-TMA transformation plays an overlooked role in aerobic marine surface waters, whereas choline-to-TMA transformation is important in anaerobic marine sediments. Our study provides new insights into the potential key microbes and metabolic pathways for TMA formation in two contrasting environments.
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影响因子：
3.6
作者：
Ansaldi, Mireille;Theraulaz, Laurence;Mejean, Vincent
通讯作者：
Mejean, Vincent
DOI：
10.1073/pnas.1215689109
发表时间：
2012-12-26
影响因子：
11.1
作者：
Craciun, Smaranda;Balskus, Emily P.
通讯作者：
Balskus, Emily P.
影响因子：
11
作者：
Lidbury, Ian D. E. A.;Murrell, J. Colin;Chen, Yin
通讯作者：
Chen, Yin
影响因子：
3.6
作者：
MEJEAN, V;IOBBINIVOL, C;PASCAL, MC
通讯作者：
PASCAL, MC
影响因子：
7.1
作者：
Flores-Mateo, Gemma;Navas-Acien, Ana;Guallar, Eliseo
通讯作者：
Guallar, Eliseo