Activation of inwardly rectifying K+ channels by GABA‐B receptors expressed in Xenopus oocytes
Activation of inwardly rectifying K+ channels by GABA‐B receptors expressed in Xenopus oocytes
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非洲爪蟾卵母细胞中表达的 GABA-B 受体激活内向整流 K+ 通道
DOI：
10.1097/00001756-199803090-00004
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发表时间：
1998
期刊：
影响因子：
1.7
通讯作者：
F. Izumi
中科院分区：
文献类型：
--
作者：
Y. Uezono;Mika Akihara;M. Kaibara;Chie Kawano;I. Shibuya;Y. Ueda;N. Yanagihara;Y. Toyohira;H. Yamashita;K. Taniyama;F. Izumi
在非洲爪哇S卵母细胞与大鼠小脑聚(A)+核糖核酸和克隆的G蛋白门控内向整流钾通道(GIRK)、GIRK1和GIRK2共同注射的卵母细胞中，GABA-B激动剂巴氯芬诱发内向整流钾电流。选择性GABA-B拮抗剂2-OH-舒氯芬和CGp 35348及GIRK抑制剂Ba2+均可抑制巴氯芬诱发的内向K+电流。相反，巴氯芬对单独注射小脑PolyA+RNA，对GIRK1或GIRK2的Poly(A)+RNA和CRNA，或仅对GIRK1和GIRK2的CRNAs的卵母细胞不产生电流。这些结果表明，大鼠小脑中的GABAB受体在非洲爪哇S卵母细胞中得到了功能性表达，并作为异源多聚体激活了由GIRK1和GIRK2组成的GIRKs。
IN Xenopu s oocytes coinjected with poly(A)+ RNA derived from the rat cerebellum and cRNAs for the cloned G protein-gated inwardly rectifying K+ channel (GIRK), GIRK1 and GIRK2, the GABA-B agonist baclofen elicited inwardly rectifying K+ currents. The inward K+ currents elicited by baclofen were inhibited by the selective GABA-B antagonists 2–OH saclofen and CGP 35348, and by the GIRK inhibitor Ba2+. In contrast, baclofen caused no currents in oocytes injected with the cerebellar poly(A)+ RNA alone, the poly(A)+ RNA and cRNA for GIRK1 or GIRK2, or only cRNAs for GIRK1 and GIRK2. These findings indicate that GABA-B receptors in the rat cerebellum were functionally expressed in Xenopu s oocytes and activated the cloned GIRKs composed of GIRK1 and GIRK2 as heteromultimers.
非洲爪蟾卵母细胞对细胞外 pH 值变化的敏感性：可能与非典型哺乳动物 GABAB 受体的表达相关。
DOI：
10.1016/0169-328x(92)90226-2
发表时间：
1992
期刊：
Brain research. Molecular brain research
影响因子：
--
作者：
Woodward,RM;Miledi,R
通讯作者：
Miledi,R
DOI：
10.1073/pnas.92.14.6542
发表时间：
1995-07-03
影响因子：
11.1
作者：
KOFUJI, P;DAVIDSON, N;LESTER, HA
通讯作者：
LESTER, HA
影响因子：
56.9
作者：
ANDRADE, R;MALENKA, RC;NICOLL, RA
通讯作者：
NICOLL, RA