Protection from COVID-19 with a VSV-based vaccine expressing the spike and nucleocapsid proteins.
Protection from COVID-19 with a VSV-based vaccine expressing the spike and nucleocapsid proteins.
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DOI：
10.3389/fimmu.2022.1025500
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
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作者：
针对新冠肺炎大流行的成功疫苗努力集中在以严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)尖峰蛋白(S)为病毒抗原的基础上，大大降低了与新冠肺炎相关的发病率和死亡率。自从这场大流行开始以来，SARS-CoV-2已经进化，导致了新的关注变种(VOC)，挑战了疫苗建立的免疫记忆。我们发现，表达SARS-CoV-2 S的水泡性口炎病毒疫苗加上保守的核衣壳蛋白(N)在金黄地鼠攻击模型中具有保护作用，分别在攻击前28天和10天单次接种。在这项研究中，只有鼻腔接种才能抵抗多重VOC的攻击，强调了N蛋白的加入确实提高了保护效果。这些数据表明，基于VSV的双抗原疫苗能够减少病毒脱落并预防SARS-CoV-2 VOC引起的疾病。
Successful vaccine efforts countering the COVID-19 pandemic are centralized around the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein as viral antigen and have greatly reduced the morbidity and mortality associated with COVID-19. Since the start of this pandemic, SARS-CoV-2 has evolved resulting in new variants of concern (VOC) challenging the vaccine-established immunologic memory. We show that vaccination with a vesicular stomatitis virus (VSV)-based vaccine expressing the SARS-CoV-2 S plus the conserved nucleocapsid (N) protein was protective in a hamster challenge model when a single dose was administered 28 or 10 days prior to challenge, respectively. In this study, only intranasal vaccination resulted in protection against challenge with multiple VOC highlighting that the addition of the N protein indeed improved protective efficacy. This data demonstrates the ability of a VSV-based dual-antigen vaccine to reduce viral shedding and protect from disease caused by SARS-CoV-2 VOC.
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影响因子：
32.4
作者：
Ferretti AP;Kula T;Wang Y;Nguyen DMV;Weinheimer A;Dunlap GS;Xu Q;Nabilsi N;Perullo CR;Cristofaro AW;Whitton HJ;Virbasius A;Olivier KJ Jr;Buckner LR;Alistar AT;Whitman ED;Bertino SA;Chattopadhyay S;MacBeath G
通讯作者：
MacBeath G
影响因子：
11.1
作者：
O'Donnell KL;Pinski AN;Clancy CS;Gourdine T;Shifflett K;Fletcher P;Messaoudi I;Marzi A
通讯作者：
Marzi A
DOI：
10.1016/j.xcrm.2020.100121
发表时间：
2020-10-20
期刊：
Cell reports. Medicine
影响因子：
--
作者：
Lee AC;Zhang AJ;Chan JF;Li C;Fan Z;Liu F;Chen Y;Liang R;Sridhar S;Cai JP;Poon VK;Chan CC;To KK;Yuan S;Zhou J;Chu H;Yuen KY
通讯作者：
Yuen KY
DOI：
10.1056/nejmoa2035389
发表时间：
2021-02-04
期刊：
The New England journal of medicine
影响因子：
--
作者：
Baden LR;El Sahly HM;Essink B;Kotloff K;Frey S;Novak R;Diemert D;Spector SA;Rouphael N;Creech CB;McGettigan J;Khetan S;Segall N;Solis J;Brosz A;Fierro C;Schwartz H;Neuzil K;Corey L;Gilbert P;Janes H;Follmann D;Marovich M;Mascola J;Polakowski L;Ledgerwood J;Graham BS;Bennett H;Pajon R;Knightly C;Leav B;Deng W;Zhou H;Han S;Ivarsson M;Miller J;Zaks T;COVE Study Group
通讯作者：
COVE Study Group
影响因子：
7.3
作者：
Hamming, I;Timens, W;van Goor, H
通讯作者：
van Goor, H