Reversible G1 arrest induced by dimethyl sulfoxide in human lymphoid cell lines: kinetics of the arrest and expression of the cell cycle marker proliferating cell nuclear antigen in Raji cells.
Reversible G1 arrest induced by dimethyl sulfoxide in human lymphoid cell lines: kinetics of the arrest and expression of the cell cycle marker proliferating cell nuclear antigen in Raji cells.
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人淋巴细胞系中二甲基亚砜诱导的可逆 G1 停滞：Raji 细胞中细胞周期标记物增殖细胞核抗原的停滞和表达的动力学。
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Kinji Tsukada
中科院分区：
文献类型：
--
作者：
Kozo Takase;Masaji Sawai;Kohtaro Yamamoto;Junichi Yata;Yoshinari Takasaki;Hirobumi Teraoka;Kinji Tsukada
为了进一步阐明可逆的细胞周期阻滞诱导Raji细胞与1.5%二甲基亚砜（DMSO）的治疗机制，我们已经进行了详细的分析动力学的逮捕和重新进入细胞周期后，去除DMSO和相关的细胞周期进展与增殖细胞核抗原（PCNA）的表达。DMSO对细胞周期模式无显著影响，通过溴脱氧尿苷预标记细胞的流式细胞术分析评估，在治疗的前19小时观察到。通过约25小时的处理检测到进入S期的明显减少;在96小时的处理后，基本上所有的细胞都被G1含量的DNA捕获。当DMSO-阻滞的细胞从阻滞中释放时，在释放后12 h开始进入S期，并以相当异步的方式持续12-14 h。在停滞的细胞中，PCNA的含量减少到约25%的量存在于增殖性生长的G1期细胞。从DMSO中释放后6 h，首先通过北方印迹法检测PCNA RNA转录物。Western blotting检测到PCNA蛋白在释放后9 h明显增加。PCNA蛋白进入细胞周期和恢复的动力学类似于静止细胞的血清刺激中所见。这些结果表明，DMSO可逆地逮捕Raji细胞在G 0或在G1期的早期点的增殖，并通过晚期G1期和进入S期的进展与PCNA基因产物的合成。
In order to elucidate further the mechanism of reversible cell cycle arrest induced by treatment of Raji cells with 1.5% dimethyl sulfoxide (DMSO), we have performed a detailed analysis of the kinetics of arrest and of reentry into the cell cycle after removal of DMSO and have correlated cell cycle progression with expression of proliferating cell nuclear antigen (PCNA). No significant effect of DMSO on cell cycle patterns, assessed by flow cytometric analysis of bromodeoxyuridine-prelabeled cells, was seen for the first 19 h of treatment. A clear reduction of entry into S phase was detected by about 25 h of treatment; essentially all cells were arrested with a G1 content of DNA after 96 h of treatment. When DMSO-arrested cells were released from the block, entry into S phase began at 12 h after release and continued in a fairly asynchronous manner for a further 12-14 h. In arrested cells, the content of PCNA was reduced to about 25% of the amount present in logarithmically growing G1 phase cells. Six h after release from DMSO, PCNA RNA transcripts were first detected by Northern blotting. The increase of PCNA protein, detected by Western blotting, was seen by 9 h after release. The kinetics of entry into the cell cycle and restoration of PCNA protein are similar to that seen in serum stimulation of quiescent cells. These results suggest that DMSO reversibly arrests proliferation of Raji cells at G0 or at an early point in G1 phase and that progression through late G1 phase and entry into S phase are correlated with synthesis of the PCNA gene product.
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DOI：
10.1073/pnas.86.13.4877
发表时间：
1989-07-01
影响因子：
11.1
作者：
LEE, SH;KWONG, AD;HURWITZ, J
通讯作者：
HURWITZ, J
人类成纤维细胞中的 DNA 修复合成需要 DNA 聚合酶 delta。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nishida,C;Reinhard,P;Linn,S
通讯作者：
Linn,S
DOI：
10.1016/s0021-9258(18)80079-5
发表时间：
1989-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Morris;M. Mathews
通讯作者：
G. Morris;M. Mathews
正常人 T 细胞细胞周期进程中 p34cdc2 及其同源物的合成调节及其与 p110Rb 磷酸化的关系。
DOI：
--
发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lucas,JJ;Terada,N;Szepesi,A;Gelfand,EW
通讯作者：
Gelfand,EW