Differential activity of plasma and vacuolar membrane transporters contributes to genotypic differences in salinity tolerance in a Halophyte Species, Chenopodium quinoa.
Differential activity of plasma and vacuolar membrane transporters contributes to genotypic differences in salinity tolerance in a Halophyte Species, Chenopodium quinoa.
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DOI：
10.3390/ijms14059267
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发表时间：
2013-04-29
影响因子：
5.6
通讯作者：
Shabala S
中科院分区：
文献类型：
--
作者：
Bonales-Alatorre E;Pottosin I;Shabala L;Chen ZH;Zeng F;Jacobsen SE;Shabala S
盐生植物物种可以作为一个非常方便的模式系统，揭示关键的离子和分子机制，赋予植物耐盐性。早些时候，我们报道了藜（藜藜），一种兼性C3盐生植物物种，通过确保大部分积累的Na+被安全地锁定在液泡中，可以有效地控制慢（SV）和快（FV）液泡膜通道的活性以匹配特定的生长条件（Bonales-Alatorre等人（2013）Plant Physiology）。本研究通过比较两种藜麦基因型的液泡膜FV和SV通道的特性，扩展了这些发现，这两种藜麦基因型在耐盐性方面存在差异。这项工作的补充，通过藜叶叶肉组织的质膜的净离子通量的动力学研究。我们的研究结果表明，多种机制有助于藜麦耐盐性的基因型差异。其中包括：（i）从叶肉中排除Na+的速率更高;（ii）维持低的细胞溶质Na+水平;（iii）叶肉中更好的K+保留;（iv）H+泵送的高速率，这增加了叶肉细胞恢复其膜电位的能力;和（v）在盐水条件下降低SV和FV通道活性的能力。这些机制似乎是高度协调的，从而使藜麦物种的显着的整体耐盐性。
Halophytes species can be used as a highly convenient model system to reveal key ionic and molecular mechanisms that confer salinity tolerance in plants. Earlier, we reported that quinoa (Chenopodium quinoa Willd.), a facultative C3 halophyte species, can efficiently control the activity of slow (SV) and fast (FV) tonoplast channels to match specific growth conditions by ensuring that most of accumulated Na+ is safely locked in the vacuole (Bonales-Alatorre et al. (2013) Plant Physiology). This work extends these finding by comparing the properties of tonoplast FV and SV channels in two quinoa genotypes contrasting in their salinity tolerance. The work is complemented by studies of the kinetics of net ion fluxes across the plasma membrane of quinoa leaf mesophyll tissue. Our results suggest that multiple mechanisms contribute towards genotypic differences in salinity tolerance in quinoa. These include: (i) a higher rate of Na+ exclusion from leaf mesophyll; (ii) maintenance of low cytosolic Na+ levels; (iii) better K+ retention in the leaf mesophyll; (iv) a high rate of H+ pumping, which increases the ability of mesophyll cells to restore their membrane potential; and (v) the ability to reduce the activity of SV and FV channels under saline conditions. These mechanisms appear to be highly orchestrated, thus enabling the remarkable overall salinity tolerance of quinoa species.
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