Genotyping of 73 UM-SCC head and neck squamous cell carcinoma cell lines.

Genotyping of 73 UM-SCC head and neck squamous cell carcinoma cell lines.
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DOI:
10.1002/hed.21198
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发表时间:
2010-04
影响因子:
2.9
通讯作者:
Carey, Thomas E.
Carey, Thomas E.
中科院分区:
医学2区
文献类型:
--
作者:
Brenner, J. Chad;Graham, Martin P.;Kumar, Bhavna;Saunders, Lindsay M.;Kupfer, Robbi;Lyons, Robert H.;Bradford, Carol R.;Carey, Thomas E.

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我们建立了多种UM-SCC(密歇根大学鳞状细胞癌)细胞系。随着时间的推移,这些已被分发到世界各地的其他实验室。最近的科学讨论指出,需要在拨款申请和期刊文章中确认细胞系的来源和身份。我们对我们收集的UM-SCC细胞系进行了基因分型,以确认其独特的身份。对早期传代的UM-SCC细胞系进行基因分型并拍照。到目前为止,对73个独特的头颈部UM-SCC细胞系(来自65个供体,包括来自17名女性的21个细胞系)进行了基因分型。在7例病例中,从同一供体建立了单独的细胞系。这些结果将发布在U of M Head and Neck SPORE Tissue Core网站上,供其他研究人员确认其实验室中使用的UM-SCC细胞具有正确的特征。使用UM-SCC细胞系的出版物应确认基因型。
We established multiple UM-SCC (University of Michigan Squamous Cell Carcinoma) cell lines. With time, these have been distributed to other labs all over the world. Recent scientific discussions have noted the need to confirm the origin and identity of cell lines in grant proposals and journal articles. We genotyped the UM-SCC cell lines in our collection to confirm their unique identity. Early passage UM-SCC cell lines were genotyped and photographed. Thus far, 73 unique head and neck UM-SCC cell lines (from 65 donors including 21 lines from 17 females) were genotyped. In 7 cases separate cell lines were established from the same donor. These results will be posted on the U of M Head and Neck SPORE Tissue Core website for other investigators to confirm that the UM-SCC cells used in their laboratories have the correct features. Publications using UM-SCC cell lines should confirm the genotype.
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发表时间: 2003-08-01
影响因子: 2.9
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