Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the Chlamydia pneumoniae inclusion membrane.
Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the Chlamydia pneumoniae inclusion membrane.
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DOI：
10.1186/1471-2180-7-38
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发表时间：
2007-05-15
期刊：
影响因子：
4.2
通讯作者：
Zhong G
中科院分区：
文献类型：
--
作者：
Luo J;Liu G;Zhong Y;Jia T;Liu K;Chen D;Zhong G
虽然已经预测了超过100种肺炎衣原体假设蛋白是包涵体膜蛋白，但是只有少数已经被实验证明是在包涵体膜中。使用融合蛋白产生的抗体，我们表征了由两个基因簇（Cpn 0146 -147和Cpn 0284 -285）编码的四个这样的假设蛋白。pneumoniae基因组。Cpn 0146和0147在包涵体膜中被检测到，而Cpn 0284和0285在包涵体内部并且主要与网状体相关，尽管所有四种蛋白都含有N-末端双叶疏水区，这是分配给包涵体膜蛋白的特征基序。这四种假设的蛋白质仅在感染C.在感染后6小时，Cpn 0147和Cpn 0146、0284和0285感染。Cpn 0146和147而不是Cpn 0284和285与宿主细胞内质网标记共定位，当通过转基因在宿主细胞胞质溶胶中表达时，已知一些衣原体包涵体膜蛋白具有这种性质。然而，C. pneumoniae包涵体膜蛋白对随后的C.肺炎感染。Cpn 0146和Cpn 0147蛋白定位于C. Cpn 0284和Cpn 0285在包涵体内，尽管所有四种都被预测为Inc蛋白，这表明需要实验表征预测的Inc蛋白。
Although more than 100 Chlamydia pneumoniae hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in the inclusion membrane. Using antibodies raised with fusion proteins, we characterized four such hypothetical proteins encoded by two gene clusters (Cpn0146-147 and Cpn0284-285) in the C. pneumoniae genome. Cpn0146 and 0147 were detected in the inclusion membrane while Cpn0284 and 0285 inside inclusion and mainly associated with reticulate bodies although all four proteins contain an N-terminal bi-lobed hydrophobic region, a signature motif assigned to inclusion membrane proteins. These four hypothetical proteins were only detected in cells infected with C. pneumoniae but not other chlamydial species, with Cpn0147 at 6 hours and Cpn0146, 0284 & 0285 at 24 hours after infection. Cpn0146 & 147 but not Cpn0284 and 285 co-localized with a host cell endoplasmic reticulum marker, a property known to be possessed by some chlamydial inclusion membrane proteins, when expressed in the host cell cytosol via transgenes. However, the endoplasmic reticulum localization of the C. pneumoniae inclusion membrane proteins did not result in inhibition of the subsequent C. pneumoniae infection. The hypothetical proteins Cpn0146 & 0147 were localized in the C. pneumoniae inclusion membrane while Cpn0284 & 0285 within the inclusion although all four were predicted to be Inc proteins, suggesting the need to experimentally characterize the predicted Inc proteins.
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影响因子：
3.6
作者：
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