The Effect of Quercetin on the Osteogenesic Differentiation and Angiogenic Factor Expression of Bone Marrow-Derived Mesenchymal Stem Cells.
The Effect of Quercetin on the Osteogenesic Differentiation and Angiogenic Factor Expression of Bone Marrow-Derived Mesenchymal Stem Cells.
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槲皮素对骨髓间充质干细胞成骨分化及血管生成因子表达的影响
DOI：
10.1371/journal.pone.0129605
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Xu Y
中科院分区：
文献类型：
--
作者：
Zhou Y;Wu Y;Jiang X;Zhang X;Xia L;Lin K;Xu Y
骨髓间充质干细胞（BMSCs）具有沿着成软骨和成骨谱系分化的能力，被广泛应用于再生医学。槲皮素作为一种传统中药，已初步报道可促进成骨细胞的成骨分化。采用MTT法、流式细胞仪（fluorescentactivatedcellsorter，FACS）、实时荧光定量PCR（real-time quantitative PCR，RT-PCR）、碱性磷酸酶（alkalinephosphatase，ALP）活性测定、钙沉积测定和酶联免疫吸附试验（enzyme-linkedimmunosorbentassay，ELISA）等方法，观察槲皮素对大鼠骨髓间充质干细胞（rBMSCs）增殖、活力、细胞形态、成骨分化和血管生成因子分泌的影响。此外，是否有丝裂原活化蛋白激酶（MAPK）信号通路参与这些过程也进行了探讨。结果表明，槲皮素能显著促进rBMSCs的增殖、成骨分化和血管生成因子的分泌，并呈剂量依赖性，2 μM浓度的槲皮素对rBMSCs增殖、成骨分化和血管生成因子分泌的促进作用最大。此外，在槲皮素处理的rBMSCs中观察到细胞外信号调节蛋白激酶（ERK）和p38通路的激活。此外，ERK抑制剂PD 98059或p38抑制剂SB 202190可分别抑制这些诱导作用。槲皮素可促进rBMSCs的增殖、成骨分化和血管生成因子的分泌，其作用机制可能与ERK和p38信号通路有关。
Bone marrow-derived mesenchymal stem cells (BMSCs) are widely used in regenerative medicine in light of their ability to differentiate along the chondrogenic and osteogenic lineages. As a type of traditional Chinese medicine, quercetin has been preliminarily reported to promote osteogenic differentiation in osteoblasts. In the present study, the effects of quercetin on the proliferation, viability, cellular morphology, osteogenic differentiation and angiogenic factor secretion of rat BMSCs (rBMSCs) were examined by MTT assay, fluorescence activated cell sorter (FACS) analysis, real-time quantitative PCR (RT-PCR) analysis, alkaline phosphatase (ALP) activity and calcium deposition assays, and Enzyme-linked immunosorbent assay (ELISA). Moreover, whether mitogen-activated protein kinase (MAPK) signaling pathways were involved in these processes was also explored. The results showed that quercetin significantly enhanced the cell proliferation, osteogenic differentiation and angiogenic factor secretion of rBMSCs in a dose-dependent manner, with a concentration of 2 μM achieving the greatest stimulatory effect. Moreover, the activation of the extracellular signal-regulated protein kinases (ERK) and p38 pathways was observed in quercetin-treated rBMSCs. Furthermore, these induction effects could be repressed by either the ERK inhibitor PD98059 or the p38 inhibitor SB202190, respectively. These data indicated that quercetin could promote the proliferation, osteogenic differentiation and angiogenic factor secretion of rBMSCs in vitro, partially through the ERK and p38 signaling pathways.
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DOI：
10.1083/jcb.200610046
发表时间：
2007-02-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ge C;Xiao G;Jiang D;Franceschi RT
通讯作者：
Franceschi RT
DOI：
10.1084/jem.174.5.1275
发表时间：
1991-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Dvorak HF;Sioussat TM;Brown LF;Berse B;Nagy JA;Sotrel A;Manseau EJ;Van de Water L;Senger DR
通讯作者：
Senger DR
影响因子：
4.8
作者：
Jadlowiec, J;Koch, H;Sfeir, C
通讯作者：
Sfeir, C
影响因子：
3.7
作者：
Hoch AI;Binder BY;Genetos DC;Leach JK
通讯作者：
Leach JK
影响因子：
14
作者：
Jiang, Xinquan;Zhao, Jun;Wang, Shaoyi;Sun, Xiaojuan;Zhang, Xiuli;Chen, Jake;Kaplan, David L.;Zhang, Zhiyuan
通讯作者：
Zhang, Zhiyuan