Mandibular repair in rats with premineralized silk scaffolds and BMP-2-modified bMSCs.
Mandibular repair in rats with premineralized silk scaffolds and BMP-2-modified bMSCs.
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DOI：
10.1016/j.biomaterials.2009.05.021
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发表时间：
2009-09
期刊：
影响因子：
14
通讯作者：
Zhang, Zhiyuan
中科院分区：
文献类型：
--
作者：
Jiang, Xinquan;Zhao, Jun;Wang, Shaoyi;Sun, Xiaojuan;Zhang, Xiuli;Chen, Jake;Kaplan, David L.;Zhang, Zhiyuan
制备了预矿化丝素蛋白支架（mSS），将生物磷灰石的骨传导特性与水溶性丝素支架（SS）结合起来，作为骨再生的复合支架。本研究的目的是评价预矿化蚕丝支架复合骨形态发生蛋白-2（BMP-2）修饰的骨髓基质细胞（bMSCs）修复大鼠下颌骨缺损的效果。体外扩增骨髓间充质干细胞，腺病毒AdBMP-2、AdLacZ基因转染骨髓间充质干细胞。然后将这些基因修饰的bMSCs与预矿化的蚕丝支架结合形成组织工程骨。用AdBMP-2转导的bMSCs/mSS构建体修复下颌骨，并与用AdLacZ转导的bMSCs/mSS构建体、天然（未转导）bMSCs/mSS构建体和单独mSS治疗的下颌骨修复进行比较。术后8周取下颌骨标本，行X线片、显微CT、组织学及免疫组化检查。BMP-2基因的存在促进了组织工程化骨的形成，并使局部骨密度（BMD）增加，表明预矿化蚕丝支架可作为bMSCs构建组织工程化骨修复下颌骨缺损的潜在基质。BMP-2基因治疗和组织工程技术可用于下颌骨的修复和骨再生。
Premineralized silk fibroin protein scaffolds (mSS) were prepared to combine the osteoconductive properties of biological apatite with aqueous-derived silk scaffold (SS) as a composite scaffold for bone regeneration. The aim of present study was to evaluate the effect of premineralized silk scaffolds combined with bone morphogenetic protein-2 (BMP-2) modified bone marrow stromal cells (bMSCs) to repair mandibular bony defects in a rat model. bMSCs were expanded and transduced with adenovirus AdBMP-2, AdLacZ gene in vitro. These genetically modified bMSCs were then combined with premineralized silk scaffolds to form tissue engineered bone. Mandibular repairs with AdBMP-2 transduced bMSCs/mSS constructs were compared with those treated with AdLacZ transduced bMSCs/mSS constructs, native (nontransduced) bMSCs/mSS constructs and mSS alone. Eight weeks post-operation, the mandibles were explanted and evaluated by radiographic observation, micro-CT, histological analysis and immunohistochemistry. The presence of BMP-2 gene enhanced tissue engineered bone in terms of the most new bone formed and the highest local bone mineral densities (BMD) found. These results demonstrated that premineralized silk scaffold could serve as a potential substrate for bMSCs to construct tissue engineered bone for mandibular bony defects. BMP-2 gene therapy and tissue engineering techniques could be used in mandibular repair and bone regeneration.
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