Mutant alleles of FAD2-1A and FAD2-1B combine to produce soybeans with the high oleic acid seed oil trait.
Mutant alleles of FAD2-1A and FAD2-1B combine to produce soybeans with the high oleic acid seed oil trait.
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DOI：
10.1186/1471-2229-10-195
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发表时间：
2010-09-09
影响因子：
5.3
通讯作者：
Bilyeu KD
中科院分区：
文献类型：
--
作者：
Pham AT;Lee JD;Shannon JG;Bilyeu KD
大豆[Glycine max（L.）梅尔。]提高大豆油的质量是大豆研究中的一个重要和不断发展的主题，以满足现代市场的营养需求和工业标准。具有高油酸的大豆油是理想的，因为这种单不饱和脂肪酸改善了油的营养和氧化稳定性。商品大豆油通常含有20%的油酸，高油酸大豆油的目标是约80%的油;以前的传统植物育种研究将油酸水平提高到油的50-60%，受到遗传复杂性和环境不稳定性的阻碍。本工作的目的是通过鉴定和组合两个δ-12脂肪酸去饱和酶基因FAD 2 -1A和FAD 2 -1B的突变来创造大豆的高油酸性状。在两个大豆品系的FAD 2 -1B等位基因中发现了三个导致错义突变的多态性。对于两个大豆品系中的每一个，在蛋白质的高度保守区域内存在一个独特的氨基酸变化。突变FAD 2 -1B等位基因与油酸水平的增加相关，尽管单独的FAD 2 -1B突变等位基因不能产生高油酸表型。当现有的FAD 2 -1A突变与新的突变FAD 2 -1B等位基因组合时，仅对于对于两种突变等位基因均为纯合的那些品系恢复高油酸表型。我们能够以两种不同的方式生产出油中含有80%油酸的传统大豆品系，每种方法只需要两个基因的贡献。所开发的高油酸大豆种质具有理想的脂肪酸组成，并且在两种生产环境下都是稳定的。FAD 2 -1B等位基因中推测的致病序列多态性被开发成用于追踪突变等位基因的高效分子标记。这里描述的用于创造高油酸大豆的资源为有效开发大豆品种以满足不断变化的市场需求提供了一个框架。
The alteration of fatty acid profiles in soybean [Glycine max (L.) Merr.] to improve soybean oil quality is an important and evolving theme in soybean research to meet nutritional needs and industrial criteria in the modern market. Soybean oil with elevated oleic acid is desirable because this monounsaturated fatty acid improves the nutrition and oxidative stability of the oil. Commodity soybean oil typically contains 20% oleic acid and the target for high oleic acid soybean oil is approximately 80% of the oil; previous conventional plant breeding research to raise the oleic acid level to just 50-60% of the oil was hindered by the genetic complexity and environmental instability of the trait. The objective of this work was to create the high oleic acid trait in soybeans by identifying and combining mutations in two delta-twelve fatty acid desaturase genes, FAD2-1A and FAD2-1B. Three polymorphisms found in the FAD2-1B alleles of two soybean lines resulted in missense mutations. For each of the two soybean lines, there was one unique amino acid change within a highly conserved region of the protein. The mutant FAD2-1B alleles were associated with an increase in oleic acid levels, although the FAD2-1B mutant alleles alone were not capable of producing a high oleic acid phenotype. When existing FAD2-1A mutations were combined with the novel mutant FAD2-1B alleles, a high oleic acid phenotype was recovered only for those lines which were homozygous for both of the mutant alleles. We were able to produce conventional soybean lines with 80% oleic acid in the oil in two different ways, each requiring the contribution of only two genes. The high oleic acid soybean germplasm developed contained a desirable fatty acid profile, and it was stable in two production environments. The presumed causative sequence polymorphisms in the FAD2-1B alleles were developed into highly efficient molecular markers for tracking the mutant alleles. The resources described here for the creation of high oleic acid soybeans provide a framework to efficiently develop soybean varieties to meet changing market demands.
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影响因子：
14.9
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