MicroRNA393 is involved in nitrogen-promoted rice tillering through regulation of auxin signal transduction in axillary buds.
MicroRNA393 is involved in nitrogen-promoted rice tillering through regulation of auxin signal transduction in axillary buds.
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MicroRNA393通过调节腋芽生长素信号转导参与氮促进水稻分蘖
DOI：
10.1038/srep32158
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发表时间：
2016-08-30
影响因子：
4.6
通讯作者：
Zhang M
中科院分区：
文献类型：
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作者：
Li X;Xia K;Liang Z;Chen K;Gao C;Zhang M
水稻分蘖对产量有重要影响，氮肥对水稻分蘖有促进作用。目前已鉴定出几个控制水稻分蘖的基因，这些基因受氮素供应不足的调控。然而，与氮素供应调控分蘖相关的分子机制知之甚少。在这里，我们报告了水稻microRNA 393（OsmiR 393）参与N-介导的分蘖，降低腋芽生长素信号的敏感性。表达分析表明，施氮使OsmiR 393基因表达上调，而两个靶基因OsAFB 2和OsTB 1表达下调;原位表达分析表明，OsmiR 393基因在腋芽分生组织中高表达。OsmiR 393过表达模拟了野生型中花11（ZH 11）中N介导的分蘖。ZH 11的OsMIR 393突变抑制了氮促进的分蘖，模拟了氮素限制的效应，且不能通过增施氮肥来恢复。Western blot分析表明，OsIAA 6在OsmiR 393过表达株系和N处理的野生型水稻中均有积累，而在OsMIR 393突变体中则有所减少。因此，我们推断，氮诱导OsmiR 393积累降低了OsTIR 1和OsAFB 2的表达，从而降低了腋芽对生长素的敏感性并稳定了OsIAA 6，从而促进了水稻分蘖。
Rice tillering has an important influence on grain yield, and is promoted by nitrogen (N) fertilizer. Several genes controlling rice tillering, which are regulated by poor N supply, have been identified. However, the molecular mechanism associated with the regulation of tillering based on N supply is poorly understood. Here, we report that rice microRNA393 (OsmiR393) is involved in N-mediated tillering by decreasing auxin signal sensitivity in axillary buds. Expression analysis showed that N fertilizer causes up-regulation of OsmiR393, but down-regulation of two target genes (OsAFB2andOsTB1).In situexpression analysis showed that OsmiR393 is highly expressed in the lateral axillary meristem. OsmiR393 overexpression mimicked N-mediated tillering in wild type Zhonghua 11 (ZH11). Mutation ofOsMIR393in ZH11 repressed N-promoted tillering, which simulated the effects of limited N, and this could not be restored by supplying N fertilizer. Western blot analysis showed that OsIAA6 was accumulated in both OsmiR393-overexpressing lines and N-treated wild type rice, but was reduced in theOsMIR393mutant. Therefore, we deduced that N-induced OsmiR393 accumulation reduces the expression ofOsTIR1andOsAFB2, which alleviates sensitivity to auxin in the axillary buds and stabilizes OsIAA6, thereby promoting rice tillering.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
OsMADS57 和 OsTB1 之间的相互作用通过 DWARF14 调节水稻分蘖
影响因子：
16.6
作者：
通讯作者：
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影响因子：
7.4
作者：
Dun, Elizabeth Ann;Ferguson, Brett James;Beveridge, Christine Anne
通讯作者：
Beveridge, Christine Anne
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
影响因子：
5.2
作者：
Jung, Harin;Lee, Dong-Keun;Kim, Ju-Kon
通讯作者：
Kim, Ju-Kon