The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14.

The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14.
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OsMADS57 和 OsTB1 之间的相互作用通过 DWARF14 调节水稻分蘖

DOI:
10.1038/ncomms2542
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发表时间:
2013
影响因子:
16.6
通讯作者:
--
中科院分区:
综合性期刊1区
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水稻分蘖是一个影响产量的多基因性状,但其调控分子模块的研究尚不清楚。本文报道了OsMADS 57与OsTB 1(TEOSINTE BRANCHED 1)相互作用并靶向D14(Dwarf 14)来控制水稻腋芽的生长。激活标记的突变体osmads 57 -1和OsMADS 57过表达系显示出增加的分蘖,而OsMADS 57反义系具有较少的分蘖。OsMIR 444 a过表达株系表现出OsMADS 57表达和分蘖抑制。此外,OsMADS 57 -1对独脚金内酯处理抑制腋芽生长不敏感,OsMADS 57的分蘖功能依赖于D14。在Osmads 57 -1中D14表达下调,而在反义和OsMIR 444 a过表达的细胞系中D14表达上调。OsMADS 57与启动子中的CArG基序[C(A/T)TTAAAAAG]结合,直接抑制D14的表达。OsMADS 57与OsTB 1的相互作用降低了OsMADS 57对D14转录的抑制。因此,0 sMIR 444 a调节的0 sMADS 57与0 sTB 1一起靶向D14以控制分蘖。这一调控机制在水稻高产分子育种中具有重要的应用价值。
Rice tillering is a multigenic trait that influences grain yield, but its regulation molecular module is poorly understood. Here we report that OsMADS57 interacts with OsTB1 (TEOSINTE BRANCHED1) and targets D14 (Dwarf14) to control the outgrowth of axillary buds in rice. An activation-tagged mutant osmads57-1 and OsMADS57-overexpression lines showed increased tillers, whereas OsMADS57 antisense lines had fewer tillers. OsMIR444a-overexpressing lines exhibited suppressed OsMADS57 expression and tillering. Furthermore, osmads57-1 was insensitive to strigolactone treatment to inhibit axillary bud outgrowth, and OsMADS57's function in tillering was dependent on D14. D14 expression was downregulated in osmads57-1, but upregulated in antisense and OsMIR444a-overexpressing lines. OsMADS57 bound to the CArG motif [C(A/T)TTAAAAAG] in the promoter and directly suppressed D14 expression. Interaction of OsMADS57 with OsTB1 reduced OsMADS57 inhibition of D14 transcription. Therefore, OsMIR444a-regulated OsMADS57, together with OsTB1, target D14 to control tillering. This regulation mechanism could have important application in rice molecular breeding programs focused on high grain yield.
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