Correlation between PPAR-α methylation level in peripheral blood and atherosclerosis of NAFLD patients with DM.
Correlation between PPAR-α methylation level in peripheral blood and atherosclerosis of NAFLD patients with DM.
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DOI：
10.3892/etm.2018.5730
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发表时间：
2018-03
影响因子：
2.7
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Ju J;Huang Q;Sun J;Zhao X;Guo X;Jin Y;Ma W;Wang W
我们研究了非酒精性脂肪性肝病（NAFLD）合并糖尿病（DM）患者外周血过氧化物酶体增殖物激活受体-α（PPAR-α）甲基化水平与动脉粥样硬化的相关性。选择山东大学齐鲁医院健康体检者50例（N组）和NAFLD合并DM患者50例（M组）。检测N、M组大鼠血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）水平。采用逆转录聚合酶链反应（PCR）和免疫荧光法检测N组和M组大鼠心肌组织中PPAR-α mRNA和蛋白的表达。采用SPSS 17. 0统计软件分析N组和M组中PPAR-α表达的差异以及PPAR-α甲基化水平与动脉粥样硬化的相关性。N、M组TC、TG、HDL、LDL差异有统计学意义（P<0.01）。苏木精-伊红染色显示M组组织病理学损害严重。PCR和免疫荧光检测结果显示N组的PPAR-α水平明显高于M组（P<0.01）。PPAR-α的异常表达与动脉粥样硬化密切相关，提示外周血中PPAR-α甲基化水平与NAFLD合并DM患者动脉粥样硬化的相关性可为NAFLD合并DM患者的诊断和治疗提供新的方向。
We investigated the correlation between the methylation levels of peroxisome proliferator-activated receptor-α (PPAR-α) in the peripheral blood and atherosclerosis in patients with nonalcoholic fatty liver disease (NAFLD) with diabetes mellitus (DM). A total of 50 normal subjects (group N) and 50 NAFLD patients with DM (group M) were selected at Qilu Hospital of Shandong University. The levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in groups N and M were detected. The mRNA and protein expressions of PPAR-α in groups N and M were detected using reverse transcription polymerase chain reaction (PCR) and immunofluorescence. The differences in expression of PPAR-α in group N and M and the correlation between PPAR-α methylation level and atherosclerosis were analyzed using SPSS17.0 statistical software. TC, TG, HDL and LDL in groups N and M were significantly different (P<0.01). Hematoxylin and eosin staining showed that the histopathological damage was severe in group M. PCR and immunofluorescence showed that PPAR-α was significantly higher in N than in group M (P<0.01). The abnormal expression of PPAR-α is closely related to atherosclerosis, indicating that the correlation between PPAR-α methylation levels in peripheral blood and atherosclerosis of NAFLD patients with DM can provide a new direction of diagnosis and treatment.
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影响因子：
2.8
作者：
Lu H;Liu H;Hu F;Zou L;Luo S;Sun L
通讯作者：
Sun L
DOI：
10.1136/bmj.d6891
发表时间：
2011-11-18
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Lazo M;Hernaez R;Bonekamp S;Kamel IR;Brancati FL;Guallar E;Clark JM
通讯作者：
Clark JM
影响因子：
4.2
作者：
Niculescu, MD;Zeisel, SH
通讯作者：
Zeisel, SH
影响因子：
15.9
作者：
Djouadi, F;Weinheimer, CJ;Kelly, DP
通讯作者：
Kelly, DP
影响因子：
15.9
作者：
Kersten, S;Seydoux, J;Wrahli, W
通讯作者：
Wrahli, W