Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer.
Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer.
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DOI：
10.1002/pros.23703
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Tang H
中科院分区：
文献类型：
--
作者：
Cui F;Hu J;Ning S;Tan J;Tang H
前列腺癌（PCa）是男性泌尿生殖系统最恶性的肿瘤之一。目前迫切需要寻找新的前列腺癌生物标志物。在本研究中，我们通过分析公共数据集（包括the cancer Genome Atlas和GSE21032）来评估MCM10在前列腺癌中的表达水平。此外，我们还进行了功能丧失试验来评估MCM10对细胞增殖、凋亡和集落形成的影响。此外，我们进行了微阵列和生物信息学分析，以探索MCM10的潜在机制。在本研究中，我们首次发现MCM10在PCa中显著上调。此外，我们发现MCM10表达的增加与晚期临床分期和高Gleason评分的PCa显著相关。Kaplan‐Meier分析显示，高MCM10表达与前列腺癌患者预后差相关。此外，功能缺失实验表明，MCM10敲低抑制细胞增殖和集落形成，但促进细胞凋亡。此外，我们进行了微阵列和生物信息学分析，发现MCM10通过调节一系列生物过程，包括癌症、细胞死亡和凋亡，来调节PCa的进展。这些结果表明MCM10可能是前列腺癌的潜在诊断和治疗靶点。
Prostate cancer (PCa) is one of the most malignant tumors of the male urogenital system. There is an urgent need to identify novel biomarkers for PCa. In this study, we evaluated the expression levels of MCM10 in prostate cancer by analyzing public datasets (including The Cancer Genome Atlas and GSE21032). Furthermore, loss of function assays was performed to evaluate the effects of MCM10 on cell proliferation, apoptosis, and colony formation. Furthermore, we performed microarray and bioinformatics analyses to explore the potential mechanisms of MCM10. In the present study, we for the first time revealed MCM10 was significantly upregulated in PCa. Moreover, we found increased MCM10 expression was significantly associated with advanced clinical stage and high Gleason score PCa. Kaplan‐Meier analysis demonstrated higher MCM10 expression was associated with a poorer patient prognosis in PCa. Furthermore, loss of function assays showed that MCM10 knockdown inhibited cell proliferation and colony formation, but promoted cell apoptosis. Additionally, we performed microarray and bioinformatics analysis and found MCM10 regulated PCa progression by regulating a series of biological processes including cancer, cell death, and apoptosis. These results suggest that MCM10 may be a potential diagnostic and therapeutic target for PCa.
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影响因子：
50.3
作者：
Taylor BS;Schultz N;Hieronymus H;Gopalan A;Xiao Y;Carver BS;Arora VK;Kaushik P;Cerami E;Reva B;Antipin Y;Mitsiades N;Landers T;Dolgalev I;Major JE;Wilson M;Socci ND;Lash AE;Heguy A;Eastham JA;Scher HI;Reuter VE;Scardino PT;Sander C;Sawyers CL;Gerald WL
通讯作者：
Gerald WL
影响因子：
--
作者：
Huang YT;Lan Q;Lorusso G;Duffey N;Rüegg C
通讯作者：
Rüegg C
影响因子：
64.5
作者：
Cancer Genome Atlas Research Network
通讯作者：
Cancer Genome Atlas Research Network
影响因子：
11.2
作者：
Su Y;Meador JA;Calaf GM;Proietti De-Santis L;Zhao Y;Bohr VA;Balajee AS
通讯作者：
Balajee AS
影响因子：
4.3
作者：
Im, Jun-Sub;Park, Soon-Young;Lee, Joon-Kyu
通讯作者：
Lee, Joon-Kyu