Antibacterial Effects of Glycyrrhetinic Acid and Its Derivatives on Staphylococcus aureus.
Antibacterial Effects of Glycyrrhetinic Acid and Its Derivatives on Staphylococcus aureus.
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DOI：
10.1371/journal.pone.0165831
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Komatsuzawa H
中科院分区：
文献类型：
--
作者：
Oyama K;Kawada-Matsuo M;Oogai Y;Hayashi T;Nakamura N;Komatsuzawa H
金黄色葡萄球菌是人类的主要病原体，由于抗生素耐药性而导致严重问题。本文研究了大黄酸及其衍生物对50株临床沙门氏菌的抗菌作用。金黄色葡萄球菌，包括18个耐甲氧西林菌株。测定了GRA、甘草次酸二钾、琥珀酰甘草次酸二钠、甘草次酸硬脂酯和甘草次酸硬脂酯对各种沙门氏菌的最低抑菌浓度（MIC）。金黄色葡萄球菌菌株。此外，我们研究了GRA和GR-SU对两种特异性S.金黄色葡萄球菌菌株。DNA微阵列分析也进行了澄清的机制GR-SU的抗菌活性。检测了5种抗菌剂对沙门氏菌的抗菌活性。金黄色葡萄球菌菌株。GRA和GR-SU显示出较强的抗菌活性相比，其他三种药物测试。在较高浓度（高于2x MIC）下，GRA和GR-SU显示出杀菌活性，而在1x MIC的浓度下，它们显示出抑菌作用。此外，GRA和GR-SU与庆大霉素表现出协同作用。通过添加GR-SU改变了大量基因（包括转运蛋白）和代谢因子（碳水化合物和氨基酸）的表达，表明这些代谢过程的抑制可能影响对碳水化合物或氨基酸的需求程度。事实上，在GRA或GR-SU的存在下，对碳水化合物或氨基酸的需求增加。GRA和GR-SU对几种沙门氏菌有较强的抗菌活性。金黄色葡萄球菌菌株，包括MRSA。这种活性可能部分是由于抑制参与碳水化合物和氨基酸代谢的几种途径。
Staphylococcus aureus is a major pathogen in humans and causes serious problems due to antibiotic resistance. We investigated the antimicrobial effect of glycyrrhetinic acid (GRA) and its derivatives against 50 clinical S. aureus strains, including 18 methicillin-resistant strains. The minimum inhibitory concentrations (MICs) of GRA, dipotassium glycyrrhizate, disodium succinoyl glycyrrhetinate (GR-SU), stearyl glycyrrhetinate and glycyrrhetinyl stearate were evaluated against various S. aureus strains. Additionally, we investigated the bactericidal effects of GRA and GR-SU against two specific S. aureus strains. DNA microarray analysis was also performed to clarify the mechanism underlying the antibacterial activity of GR-SU. We detected the antimicrobial activities of five agents against S. aureus strains. GRA and GR-SU showed strong antibacterial activities compared to the other three agents tested. At a higher concentration (above 2x MIC), GRA and GR-SU showed bactericidal activity, whereas at a concentration of 1x MIC, they showed a bacteriostatic effect. Additionally, GRA and GR-SU exhibited a synergistic effect with gentamicin. The expression of a large number of genes (including transporters) and metabolic factors (carbohydrates and amino acids) was altered by the addition of GR-SU, suggesting that the inhibition of these metabolic processes may influence the degree of the requirement for carbohydrates or amino acids. In fact, the requirement for carbohydrates or amino acids was increased in the presence of either GRA or GR-SU. GRA and GR-SU exhibited strong antibacterial activity against several S. aureus strains, including MRSA. This activity may be partly due to the inhibition of several pathways involved in carbohydrate and amino acid metabolism.
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影响因子：
4.2
作者：
Iino, S;Tango, T;Suzuki, H
通讯作者：
Suzuki, H
影响因子：
7.3
作者：
INOUE, H;MORI, T;KOSHIHARA, Y
通讯作者：
KOSHIHARA, Y
影响因子：
3.3
作者：
INOUE, H;INOUE, K;SHIBATA, S
通讯作者：
SHIBATA, S
影响因子：
3.6
作者：
Komatsuzawa, H;Fujiwara, T;Sugai, M
通讯作者：
Sugai, M
影响因子：
2.7
作者：
CORTHOUT, J;PIETERS, L;VLIETINCK, A
通讯作者：
VLIETINCK, A