GABAergic signaling induces divergent neuronal Ca2+ responses in the suprachiasmatic nucleus network.
GABAergic signaling induces divergent neuronal Ca2+ responses in the suprachiasmatic nucleus network.
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DOI：
10.1111/j.1460-9568.2009.06944.x
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发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Allen CN
中科院分区：
文献类型：
--
作者：
Irwin RP;Allen CN
γ-氨基丁酸（GABA）能的视交叉上核（SCN）神经元之间的细胞间通讯促进SCN网络内单个神经振荡器的光诱导相变和同步化。我们使用比率Ca 2+成像技术来记录细胞内钙浓度（[Ca 2 +]i）的变化，以研究GABA在神经元间通讯中的作用以及SCN神经元网络对模拟夹带光信号的视神经刺激的反应。刺激视网膜下丘脑束（RHT）引起不同的钙离子反应的神经元，不同区域内的SCN与模式，与那些诱发的药理GABA应用程序。GABAA和GABAB受体激动剂和拮抗剂用于评估GABA诱导的[Ca 2 +]i变化的组分。应用GABAA受体拮抗剂gabazine诱导的变化在基线[Ca 2 +]i的方向相反，诱发GABA，并同样改变了RHT刺激诱导的Ca 2+反应。GABA的应用引起的Ca 2+反应在SCN网络的时间和区域不同。NKCC 1协同转运蛋白阻断剂布美他尼和L型钙通道阻断剂尼莫地平可减弱GABA诱导的[Ca 2 +]i升高。这些结果表明，生理GABA诱导相反的影响[Ca 2 +]i的基础上的氯平衡电位，并可能在SCN网络的神经元Ca 2+平衡，同步和光输入信号的调制中发挥重要作用。
Intercellular communication between γ-aminobutyric acid (GABA)ergic suprachiasmatic nucleus (SCN) neurons facilitates light-induced phase changes and synchronization of individual neural oscillators within the SCN network. We used ratiometric Ca2+ imaging techniques to record changes in the intracellular calcium concentration ([Ca2+]i) to study the role of GABA in interneuronal communication and the response of the SCN neuronal network to optic nerve stimulations that mimic entraining light signals. Stimulation of the retinohypothalamic tract (RHT) evoked divergent Ca2+ responses in neurons that varied regionally within the SCN with a pattern that correlated with those evoked by pharmacological GABA applications. GABAA and GABAB receptor agonists and antagonists were used to evaluate components of the GABA-induced changes in [Ca2+]i. Application of the GABAA receptor antagonist gabazine induced changes in baseline [Ca2+]i in a direction opposite to that evoked by GABA, and similarly altered the RHT stimulation-induced Ca2+ response. GABA application induced Ca2+ responses varied in time and region within the SCN network. The NKCC1 cotransporter blocker, bumetanide, and L-type calcium channel blocker, nimodipine, attenuated the GABA-induced rise of [Ca2+]i. These results suggest that physiological GABA induces opposing effects on [Ca2+]i based on the chloride equilibrium potential, and may play an important role in neuronal Ca2+ balance, synchronization and modulation of light input signaling in the SCN network.
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