Differential disease resistance response in the barley necrotic mutant nec1.
Differential disease resistance response in the barley necrotic mutant nec1.
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DOI：
10.1186/1471-2229-11-66
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发表时间：
2011-04-15
影响因子：
5.3
通讯作者：
Rostoks N
中科院分区：
文献类型：
--
作者：
Keisa A;Kanberga-Silina K;Nakurte I;Kunga L;Rostoks N
虽然离子通量被认为是在对病原体的反应过程中的信号转导的一个组成部分，只有少数离子通道是已知的参与植物对感染的反应。CNGC 4是一种抗病相关的环核苷酸门控离子通道。拟南芥CNGC 4突变体hlm 1和dnd 2显示受损的过敏反应（HR），生长迟缓，组成型活性水杨酸（SA）介导的病程相关的反应和提高对细菌病原体的抗性。大麦CNGC 4与AtCNGC 4具有67%的氨基酸同源性。由CNGC 4移码突变组成的大麦突变体nec 1表现出坏死表型并组成性过表达PR-1，但不知道nec 1突变对大麦对不同类型病原体的抗性有什么影响。nec 1突变体积累了大量的SA和过氧化氢相比，亲本cv。帕克兰。研究nec 1抗病性的实验表明，nec 1突变对针对丁香假单胞菌致病变种的非宿主抗性具有积极影响。番茄（Pst）在高接种密度，而在正常Pst接种浓度nec 1抗性没有不同的wt.与增强的P. gramingae抗性相反，对活体营养真菌Blumeria graminis f.小麦白粉病的病原菌大麦专化型（Bgh）在nec 1中没有发生改变。nec 1突变体显著过表达小种非特异性Bgh抗性相关基因BI-1和MLO。BI-1和MLO的诱导表明nec 1参与了小种非特异性Bgh抗性，因此评估了nec 1对mlo-5介导的Bgh抗性的影响。nec 1/mlo-5双突变体对Bgh的抗性与nec 1/mlo-5植株相同，表明nec 1不损害mlo-5小种的非特异Bgh抗性。总之，结果表明，nec 1突变改变激活系统获得的抗性相关的生理标记和非寄主抗性大麦，而不改变快速本地化的反应过程中与宿主病原体的兼容相互作用。nec 1对非宿主病原菌Pst的抗性增加表明，nec 1突变可能会影响大麦抗病性的某些方面，但仍有待确定，如果对抗病性的影响是SA信号变化的直接反应。
Although ion fluxes are considered to be an integral part of signal transduction during responses to pathogens, only a few ion channels are known to participate in the plant response to infection. CNGC4 is a disease resistance-related cyclic nucleotide-gated ion channel. Arabidopsis thaliana CNGC4 mutants hlm1 and dnd2 display an impaired hypersensitive response (HR), retarded growth, a constitutively active salicylic acid (SA)-mediated pathogenesis-related response and elevated resistance against bacterial pathogens. Barley CNGC4 shares 67% aa identity with AtCNGC4. The barley mutant nec1 comprising of a frame-shift mutation of CNGC4 displays a necrotic phenotype and constitutively over-expresses PR-1, yet it is not known what effect the nec1 mutation has on barley resistance against different types of pathogens. nec1 mutant accumulated high amount of SA and hydrogen peroxide compared to parental cv. Parkland. Experiments investigating nec1 disease resistance demonstrated positive effect of nec1 mutation on non-host resistance against Pseudomonas syringae pv. tomato (Pst) at high inoculum density, whereas at normal Pst inoculum concentration nec1 resistance did not differ from wt. In contrast to augmented P. syringae resistance, penetration resistance against biotrophic fungus Blumeria graminis f. sp. hordei (Bgh), the causal agent of powdery mildew, was not altered in nec1. The nec1 mutant significantly over-expressed race non-specific Bgh resistance-related genes BI-1 and MLO. Induction of BI-1 and MLO suggested putative involvement of nec1 in race non-specific Bgh resistance, therefore the effect of nec1on mlo-5-mediated Bgh resistance was assessed. The nec1/mlo-5 double mutant was as resistant to Bgh as Nec1/mlo-5 plants, suggesting that nec1 did not impair mlo-5 race non-specific Bgh resistance. Together, the results suggest that nec1 mutation alters activation of systemic acquired resistance-related physiological markers and non-host resistance in barley, while not changing rapid localized response during compatible interaction with host pathogen. Increased resistance of nec1 against non-host pathogen Pst suggests that nec1 mutation may affect certain aspects of barley disease resistance, while it remains to be determined, if the effect on disease resistance is a direct response to changes in SA signaling.
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DOI：
10.1094/mpmi-21-10-1285
发表时间：
2008-10
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Genger RK;Jurkowski GI;McDowell JM;Lu H;Jung HW;Greenberg JT;Bent AF
通讯作者：
Bent AF
影响因子：
4.3
作者：
Jimenez, A;Creissen, G;Mullineaux, P
通讯作者：
Mullineaux, P
影响因子：
7.2
作者：
Jirage, D;Zhou, N;Glazebrook, J
通讯作者：
Glazebrook, J
影响因子：
11.6
作者：
Balagué, C;Lin, BQ;Roby, D
通讯作者：
Roby, D
影响因子：
64.8
作者：
Collins, NC;Thordal-Christensen, H;Schulze-Lefert, P
通讯作者：
Schulze-Lefert, P