Site-directed photochemical disruption of the actin cytoskeleton by actin-binding Rose Bengal-conjugates.

Site-directed photochemical disruption of the actin cytoskeleton by actin-binding Rose Bengal-conjugates.
复制标题

通过与肌动蛋白结合的玫瑰红缀合物对肌动蛋白细胞骨架进行定点光化学破坏。

DOI:
10.1016/s1011-1344(02)00382-2
复制
发表时间:
2002
期刊:
Journal of photochemistry and photobiology. B, Biology
影响因子:
--
通讯作者:
Berrios,Miguel
Berrios,Miguel
中科院分区:
--
文献类型:
--
作者:
Conlon,KimberlyA;Rosenquist,Thomas;Berrios,Miguel

文献摘要

参考文献

被引文献

相似文献

原位光诱导,非酶消化的细胞骨架肌动蛋白的咕吨染料共轭重肌球蛋白,抗肌动蛋白抗体和/或抗肌球蛋白抗体的报告。将染料虎红与抗肌动蛋白抗体、抗肌球蛋白抗体或重肌球蛋白结合。在我们的实验条件下,可见光诱导的非酶分解的细胞骨架肌动蛋白时,哺乳动物组织培养细胞进行探测,无论是与虎红共轭抗肌动蛋白和/或抗肌球蛋白抗体。在用可见光照射之前,用虎红偶联的重酶解肌球蛋白探测组织培养细胞时,获得了类似的结果。原位光化学反应依赖于肌动蛋白结合虎红结合物的存在。
The in situ light-induced, non-enzymatic digestion of cytoskeletal actin by a xanthene dye conjugated to heavy meromyosin, anti-actin antibodies and/or anti-myosin antibodies is reported. The dye Rose Bengal was conjugated to either anti-actin antibodies, anti-myosin antibodies or heavy meromyosin. Under our experimental conditions, visible light induced the non-enzymatic breakdown of cytoskeletal actin when mammalian tissue culture cells were probed either with Rose Bengal-conjugated anti-actin and/or anti-myosin antibodies. Similar results were obtained when tissue culture cells were probed with Rose Bengal-conjugated heavy meromyosin before irradiation with visible light. The in situ photochemical reaction depended on the presence of actin-binding Rose Bengal-conjugates.
DOI: 10.1016/s1011-1344(01)00241-x
发表时间: 2001-12
期刊: Journal of photochemistry and photobiology. B, Biology
影响因子: --
作者:
K. Conlon;M. Berrios
通讯作者: K. Conlon;M. Berrios
营养缺乏的哺乳动物组织中 8-氧代鸟嘌呤的免疫定位
DOI: 10.1179/his.2000.23.1.37
发表时间: 2000
影响因子: 1.1
作者:
Kimberly A. Conion;A. Grollman;M. Berrios
通讯作者: M. Berrios
果蝇中的无细胞核组装和拆卸。
DOI: 10.1016/s0091-679x(08)60888-2
发表时间: 1998
影响因子: --
作者:
P. Fisher;M. Berrios
通讯作者: M. Berrios
DOI: 10.1016/s0167-4838(98)00145-9
发表时间: 1998-09-08
期刊: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
影响因子: --
作者:
Buranaprapuk, A;Leach, SP;Bocarsly, JR
通讯作者: Bocarsly, JR
培养的成纤维细胞中机械化学蛋白质的细胞内分布。
影响因子: 11.1
作者:
M. Heggeness;K. Wang;S. Singer
通讯作者: S. Singer