Pentatricopeptide repeat proteins involved in plant organellar RNA editing.

Pentatricopeptide repeat proteins involved in plant organellar RNA editing.
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DOI:
10.4161/rna.24908
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发表时间:
2013
期刊:
影响因子:
4.1
通讯作者:
Nakamura T
Nakamura T
中科院分区:
生物学3区
文献类型:
--
作者:
Yagi Y;Tachikawa M;Noguchi H;Satoh S;Obokata J;Nakamura T

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C-to-U RNA编辑广泛存在于陆生植物的细胞器RNA中。最近的研究揭示了植物特有的五肽重复(PPR)蛋白家族对植物线粒体和叶绿体中的RNA编辑和其他RNA加工事件的重要性。PPR蛋白是一种序列特异的RNA结合蛋白,它识别特定的C残基以供编辑。PPR基序的RNA识别密码的发现,包括对单个RNA编辑位点及其对应的PPR蛋白的验证和预测,扩大了我们对PPR蛋白在植物细胞器RNA编辑中的分子功能的理解。利用这一知识和共表达数据库,我们已经鉴定出两个新的PPR蛋白,它们介导叶绿体RNA编辑。此外,使用PPR RNA识别码的计算靶标分配表明了一种独特的、未知的作用模式,通过这种模式,PPR蛋白在RNA编辑中起到了位置识别以外的功能。
C-to-U RNA editing has been widely observed in organellar RNAs in terrestrial plants. Recent research has revealed the significance of a large, plant-specific family of pentatricopeptide repeat (PPR) proteins for RNA editing and other RNA processing events in plant mitochondria and chloroplasts. PPR protein is a sequence-specific RNA-binding protein that identifies specific C residues for editing. Discovery of the RNA recognition code for PPR motifs, including verification and prediction of the individual RNA editing site and its corresponding PPR protein, expanded our understanding of the molecular function of PPR proteins in plant organellar RNA editing. Using this knowledge and the co-expression database, we have identified two new PPR proteins that mediate chloroplast RNA editing. Further, computational target assignment using the PPR RNA recognition codes suggests a distinct, unknown mode-of-action, by which PPR proteins serve a function beyond site recognition in RNA editing.
DOI: 10.1073/pnas.0307163101
发表时间: 2004-01-06
影响因子: 11.1
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