The interactions of dendritic cells with osteoblasts on titanium surfaces: an in vitro investigation
The interactions of dendritic cells with osteoblasts on titanium surfaces: an in vitro investigation
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钛表面树突状细胞与成骨细胞的相互作用：体外研究
DOI：
10.1007/s00784-019-02852-w
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发表时间：
2019-03
影响因子：
3.4
通讯作者：
Zhang Xiaoxin
中科院分区：
文献类型：
--
作者：
Yang Yang;Wang Xuzhu;Miron Richard J;Zhang Xiaoxin
目的骨免疫相互作用在材料与宿主的结合中具有重要作用。树突状细胞（Dendritic cells，DCs）是骨免疫细胞家族中最常见的成员。牙种植体的钛表面倾向于促进成熟的树突状细胞表型，增加促炎分泌。然而，这种微环境对植入物周围细胞，特别是成骨细胞的行为的影响，以及组织整合如何在这种生物材料表面上发生，人们知之甚少。因此，本研究旨在探讨树突状细胞与成骨细胞在钛表面的相互作用。DC接种在PT和SLA钛表面进行比较的增殖，表面标志物，和炎症基因的expressions.Materials和MethodsDC接种在PT和SLA钛表面进行比较的增殖，表面标志物，和炎症基因的表达。结果PT和SLA表面接种的DC表达CD86和促炎细胞因子，而抗炎细胞因子表达减少。钛表面的CM抑制成骨细胞分化，降低成骨基因RUNX 2、COL 1、ALP和OCN的表达，降低ALP活性以及茜素红染色。结论这些发现表明钛表面使DC转向成熟表型，从而抑制成骨细胞的分化和矿化。临床意义了解免疫细胞对成骨行为的显着影响，减少免疫反应的努力可能具有临床意义。良好的免疫环境可以提高种植体的存活率。
ObjectivesOsteoimmune interactions possess a critical part in the integration of materials and hosts. Dendritic cells (DCs) are the most common members of osteoimmune cells family. The titanium surfaces of dental implants tend to promote a mature dendritic cell phenotype with increased proinflammatory secretion. However, very little is known to the effects of this microenvironment on the behaviors of cells around implants, especially osteoblasts, and how the tissue integrations take place on such biomaterial surfaces. Therefore, the present study was to investigate the interactions of DCs with osteoblasts on titanium surfaces. DCs seeded on PT and SLA titanium surfaces were compared by assays for the proliferations, surface markers, and inflammatory genes expressions.Materials and methodsDCs seeded on PT and SLA titanium surfaces were compared by assays for the proliferations, surface markers, and inflammatory genes expressions. Next, we harvested the dendritic cell–conditioned medium (CM) and investigated the effects of CM on MC3T3-E1.ResultsThe results showed an increase in CD86 and proinflammatory expressions of DCs seeded on PT and SLA surfaces, as well as a decrease in anti-inflammatory cytokines. The CM from titanium surfaces inhibited the osteoblast differentiation with reduced expression of osteogenic genes RUNX2, COL1, ALP, and OCN and decreased ALP activity as well as Alizarin red staining.ConclusionThese findings suggested that titanium surfaces switch DCs toward maturation phenotypes and thus inhibit the differentiation and mineralization of osteoblasts.Clinical relevanceKnowing the significant impact of immune cells on osteogenesis behaviors, some efforts to decrease the immune reaction might be of clinical significance. Favorable immune environments can increase the dental implants survival rate in patients.
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影响因子：
4.6
作者：
Athanasou NA
通讯作者：
Athanasou NA
影响因子：
2.9
作者：
Shuang Y;Yizhen L;Zhang Y;Fujioka-Kobayashi M;Sculean A;Miron RJ
通讯作者：
Miron RJ
影响因子：
6.2
作者：
Alnaeeli, Mawadda;Park, Jaekweon;Teng, Yen-Tung A.
通讯作者：
Teng, Yen-Tung A.
影响因子：
4.3
作者：
Cochran, DL;Buser, D;Simpson, JP
通讯作者：
Simpson, JP
影响因子：
9.7
作者：
Cheng, Hao;Xiong, Wei;Li, Feng
通讯作者：
Li, Feng