A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster.
A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster.
复制标题
使用果蝇测量奖励寻求行为改变的简单方法。
DOI：
10.3791/54910
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
G. Shohat
中科院分区：
文献类型：
--
作者：
Shir Zer;Julia Ryvkin;H. Wilner;H. Zak;A. Shmueli;G. Shohat
我们描述了一个协议，用于测量乙醇自我管理果蝇（果蝇）作为奖励状态变化的代理。我们展示了一种简单的方法来利用苍蝇奖励系统，修改与自然奖励相关的经验，并使用自愿乙醇消费作为奖励状态变化的衡量标准。该方法作为一个相关的工具来研究在经验介导的内部状态变化中发挥作用的神经元和基因。该协议是由两个独立的部分：暴露的苍蝇奖励和非奖励的经验，并测定自愿乙醇消费作为衡量的动机，以获得药物奖励。这两个部分可以独立地用于诱导经验的调节，分别作为进一步下游测定的初始步骤或作为独立的二选一饲养测定。该方案不需要复杂的设置，因此可以应用于任何具有基本苍蝇培养工具的实验室。
We describe a protocol for measuring ethanol self-administration in fruit flies (Drosophila melanogaster) as a proxy for changes in reward states. We demonstrate a simple way to tap into the fly reward system, modify experiences related to natural reward, and use voluntary ethanol consumption as a measure for changes in reward states. The approach serves as a relevant tool to study the neurons and genes that play a role in experience-mediated changes of internal state. The protocol is composed of two discrete parts: exposing the flies to rewarding and nonrewarding experiences, and assaying voluntary ethanol consumption as a measure of the motivation to obtain a drug reward. The two parts can be used independently to induce the modulation of experience as an initial step for further downstream assays or as an independent two-choice feeding assay, respectively. The protocol does not require a complicated setup and can therefore be applied in any laboratory with basic fly culture tools.
影响因子：
3.7
作者：
Ro J;Harvanek ZM;Pletcher SD
通讯作者：
Pletcher SD