FLIC: high-throughput, continuous analysis of feeding behaviors in Drosophila.
FLIC: high-throughput, continuous analysis of feeding behaviors in Drosophila.
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DOI：
10.1371/journal.pone.0101107
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Pletcher SD
中科院分区：
文献类型：
--
作者：
Ro J;Harvanek ZM;Pletcher SD
我们提出了一个完整的硬件和软件系统，用于收集和量化连续测量的果蝇，果蝇的摄食行为。FLIC（Fly Liquid-Food Interaction Counter，苍蝇液体-食物相互作用计数器）检测苍蝇与液体食物发生物理接触时产生的短至50 µs的模拟电子信号。信号特征有效地区分不同类型的行为，如喂食和品尝事件。FLIC系统的性能与用于简单测定的流行方法一样好或更好，它为研究摄食行为的新成分提供了前所未有的机会，例如食物偏好的时间依赖性变化以及个体的动机和饥饿水平。此外，FLIC实验可以无限期地持续不受干扰，我们强调这种能力，通过建立一个详细的图片的昼夜摄食行为的苍蝇。我们相信FLIC系统将与现代分子技术携手合作，以促进利用现代高通量技术对果蝇摄食行为的机制研究。
We present a complete hardware and software system for collecting and quantifying continuous measures of feeding behaviors in the fruit fly, Drosophila melanogaster. The FLIC (Fly Liquid-Food Interaction Counter) detects analog electronic signals as brief as 50 µs that occur when a fly makes physical contact with liquid food. Signal characteristics effectively distinguish between different types of behaviors, such as feeding and tasting events. The FLIC system performs as well or better than popular methods for simple assays, and it provides an unprecedented opportunity to study novel components of feeding behavior, such as time-dependent changes in food preference and individual levels of motivation and hunger. Furthermore, FLIC experiments can persist indefinitely without disturbance, and we highlight this ability by establishing a detailed picture of circadian feeding behaviors in the fly. We believe that the FLIC system will work hand-in-hand with modern molecular techniques to facilitate mechanistic studies of feeding behaviors in Drosophila using modern, high-throughput technologies.
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DOI：
10.1073/pnas.0702726104
发表时间：
2007-05-15
影响因子：
11.1
作者：
Ja, William W.;Carvalho, Gil B.;Benzer, Seymour
通讯作者：
Benzer, Seymour
卡路里不能通过果蝇饮食限制来解释寿命的延长。
影响因子：
9.8
作者：
Mair, William;Piper, Matthew D W;Partridge, Linda
通讯作者：
Partridge, Linda
影响因子：
64.8
作者：
HARDIN, PE;HALL, JC;ROSBASH, M
通讯作者：
ROSBASH, M
DOI：
10.1111/j.1749-6632.1969.tb12939.x
发表时间：
1969-01-01
影响因子：
5.2
作者：
JACOBS, HL;SHARMA, KN
通讯作者：
SHARMA, KN
影响因子：
7.8
作者：
Skorupa, Danielle A.;Dervisefendic, Azra;Pletcher, Scott D.
通讯作者：
Pletcher, Scott D.