Immunohistochemical demonstration of human carbonic anhydrase isoenzyme II in brain tumours
Immunohistochemical demonstration of human carbonic anhydrase isoenzyme II in brain tumours
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人碳酸酐酶同工酶 II 在脑肿瘤中的免疫组织化学演示
DOI：
10.1007/bf02389687
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
H. Rajaniemi
中科院分区：
文献类型：
--
作者：
A. Parkkila;R. Herva;S. Parkkila;H. Rajaniemi
碳酸酐酶（carbonic anhydrase，CA）是中枢神经系统（central nervous system，CNS）中一种重要的酶，参与调节脑脊髓液（cerebral fluid，CSF）的生成和酸碱平衡。同工酶II（CA II）是CNS中分布最广泛的CA，至少存在于髓鞘、少突胶质细胞、星形胶质细胞和脉络丛中。这项研究是进行检查CA II在不同的脑肿瘤从31例患者的存在。识别CA II的特异性抗体用于肿瘤标本的免疫过氧化物酶染色。以抗CA I、VI血清和正常兔血清为对照。在所有星形细胞肿瘤（n = 9）、少突胶质瘤（n = 3）和髓母细胞瘤（n = 3）中均观察到CA II阳性染色。大多数恶性肿瘤表现出最强的染色。此外，四个听神经鞘瘤，一个丛状神经纤维瘤，脉络丛乳头状瘤，一个室管膜母细胞瘤和室管膜下瘤表达的酶。脑膜瘤（n = 4）和神经元肿瘤（N = 4），包括1例小脑发育不良性神经节细胞瘤（Lhermitte-Duclos），均为阴性。抗CA I、VI和正常兔血清在肿瘤细胞中无特异性染色。星形细胞瘤中CA II的存在通过Western印迹法证实，其揭示了对应于CA II的独特的29 kDa多肽带。抗CA I血清也显示出类似的单一29 kDa条带，识别红细胞中大量存在的酶。本研究结果表明，尽管恶性转化的细胞，CA II的表达是持续在星形细胞肿瘤，少突胶质细胞瘤，室管膜和脉络丛肿瘤和肿瘤的神经鞘细胞起源。我们的研究结果表明，一些肿瘤含有丰富的CA II，这可能会泄漏到CSF中。
SummaryCarbonic anhydrase (CA) is a functionally important enzyme in the central nervous system (CNS), where it is involved in the control of the acid-base balance and regulates the production of cerebrospinal fluid (CSF). Isoenzyme II (CA II) is the most widely distributed CA in the CNS, being present in at least myelin, oligodendrocytes, astrocytes and the choroid plexus. This study was undertaken to examine the presence of CA II in different brain tumours from 31 patients. Specific antibodies recognizing CA II were used in immunoperoxidase staining of tumour specimens. Anti-CA I and VI sera and normal rabbit serum were used as controls. CA II-positive staining was observed in all the astrocytic tumours (n = 9), oligodendrogliomas (n = 3) and medulloblastomas (n = 3). The most malignant tumours exhibited the strongest staining. In addition, four acoustic neurinomas, one plexiform neurofibroma, one choroid plexus papilloma, one ependymoblastoma and one subependymoma expressed the enzyme. Meningiomas (n = 4) and neuronal tumours (N = 4), including one dysplastic gangliocytoma of the cerebellum (Lhermitte-Duclos), were negative. Anti-CA I, VI and normal rabbit sera showed no specific staining in tumour cells. The presence of CA II in the astrocytomas was confirmed by Western blotting, which revealed a distinct 29 kDa polypeptide band corresponding the CA II. Anti-CA I serum showed similarly a single 29 kDa band, recognizing the enzyme which is abundantly present in the erythrocytes. The present results demonstrate that despite the malignant transformation of the cells, the expression of CA II is sustained in astrocytic tumours, oligodendrogliomas, ependymal and choroid plexus tumours and tumours of nerve sheath cell origin. Our results suggest that some tumours contain abundant CA II, which might leak into the CSF.
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DOI：
10.1016/s0021-9258(19)75797-4
发表时间：
1987-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
H. Murakami;W. Sly
通讯作者：
H. Murakami;W. Sly
大鼠唾液中碳酸酐酶的纯化和表征。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Feldstein,JB;Silverman,DN
通讯作者：
Silverman,DN
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Whitney,PL;Briggle,TV
通讯作者：
Briggle,TV
DOI：
10.1073/pnas.89.15.6823
发表时间：
1992-08-01
影响因子：
11.1
作者：
GHANDOUR, MS;LANGLEY, OK;SLY, WS
通讯作者：
SLY, WS