A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters.
A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters.
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重组刺突蛋白亚单位疫苗赋予仓鼠针对 SARS-CoV-2 感染和传播的保护性免疫力
DOI：
10.1126/scitranslmed.abg1143
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发表时间：
2021-08-11
影响因子：
17.1
通讯作者：
Xia, Ningshao
中科院分区：
文献类型：
--
作者：
Wu, Yangtao;Huang, Xiaofen;Yuan, Lunzhi;Wang, Shaojuan;Zhang, Yali;Xiong, Hualong;Chen, Rirong;Ma, Jian;Qi, Ruoyao;Nie, Meifeng;Xu, Jingjing;Zhang, Zhigang;Chen, Liqiang;Wei, Min;Zhou, Ming;Cai, Minping;Shi, Yang;Zhang, Liang;Yu, Huan;Hong, Junping;Wang, Zikang;Hong, Yunda;Yue, Mingxi;Li, Zonglin;Chen, Dabing;Zheng, Qingbing;Li, Shaowei;Chen, Yixin;Cheng, Tong;Zhang, Jun;Zhang, Tianying;Zhu, Huachen;Zhao, Qinjian;Yuan, Quan;Guan, Yi;Xia, Ningshao
SARS-CoV-2刺突蛋白亚单位疫苗诱导体液和细胞免疫反应，保护仓鼠免受感染并防止传播。应对传播严重急性呼吸综合征冠状病毒2(SARS-CoV-2)疫苗的一个重要特征是疫苗是否能防止传播给其他人。在本研究中，Wu et al.用SARS-CoV-2尖峰蛋白亚单位疫苗StriFK和名为FH002C的氮二磷酸盐修饰的锌铝混合佐剂接种小鼠、仓鼠和食蟹猴。该疫苗在所有三种模型中都能诱导抗体和细胞免疫。StriFK-FH002C疫苗接种可以防止仓鼠将病毒传播给未接种疫苗的共居仓鼠。这与接种疫苗的仓鼠在攻击后上呼吸道病毒载量较低有关。因此，StriFK-FH002C代表了SARS-CoV-2的有效候选疫苗。需要多种安全有效的疫苗来激发对严重急性呼吸综合征冠状病毒2(SARS-CoV-2)的免疫反应，以应对正在进行的2019年冠状病毒病(新冠肺炎)大流行。在这里，我们开发了一种蛋白质亚单位疫苗，该疫苗由刺激性胞外结构域蛋白(StriFK)和氮二磷酸盐修饰的锌铝杂交佐剂(FH002C)组成。StriFK-FH002C在小鼠、仓鼠和食蟹猴身上产生的中和抗体效价显著高于从新冠肺炎恢复期个体分离的血浆中观察到的中和抗体效价。StriFK-FH002C还在小鼠中诱导了TH1和TH2极化的辅助T细胞反应。在仓鼠中，StriFK-FH002C免疫保护动物免受SARS-CoV-2攻击，表现为没有病毒诱导的体重减轻，疾病症状减少，肺部病理减少。给仓鼠接种StriFK-FH002C疫苗也减少了未接种疫苗的共居仓鼠在笼内的病毒传播。综上所述，StriFK-FH002C代表了一种有效的、基于蛋白质亚单位的SARS-CoV-2候选疫苗。
A SARS-CoV-2 spike protein subunit vaccine induced humoral and cellular immune responses that protected hamsters against infection and prevented transmission. Tackling transmission An important feature of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is whether or not the vaccine prevents transmission to others. In this study, Wu et al. vaccinated mice, hamsters, and cynomolgus monkeys with a SARS-CoV-2 spike protein subunit vaccine, StriFK, plus a nitrogen bisphosphonate–modified zinc-aluminum hybrid adjuvant called FH002C. The vaccine elicited antibody and cell-mediated immunity in all three models. StriFK-FH002C vaccination prevented hamsters from transmitting virus to unvaccinated, cohoused hamsters. This was associated with lower viral load in the upper respiratory tract of vaccinated hamsters after challenge. Thus, StriFK-FH002C represents an effective vaccine candidate for SARS-CoV-2. Multiple safe and effective vaccines that elicit immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are necessary to respond to the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here, we developed a protein subunit vaccine composed of spike ectodomain protein (StriFK) plus a nitrogen bisphosphonate–modified zinc-aluminum hybrid adjuvant (FH002C). StriFK-FH002C generated substantially higher neutralizing antibody titers in mice, hamsters, and cynomolgus monkeys than those observed in plasma isolated from COVID-19 convalescent individuals. StriFK-FH002C also induced both TH1- and TH2-polarized helper T cell responses in mice. In hamsters, StriFK-FH002C immunization protected animals against SARS-CoV-2 challenge, as shown by the absence of virus-induced weight loss, fewer symptoms of disease, and reduced lung pathology. Vaccination of hamsters with StriFK-FH002C also reduced within-cage virus transmission to unvaccinated, cohoused hamsters. In summary, StriFK-FH002C represents an effective, protein subunit–based SARS-CoV-2 vaccine candidate.
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影响因子：
56.9
作者：
Hsieh, Ching-Lin;Goldsmith, Jory A.;McLellan, Jason S.
通讯作者：
McLellan, Jason S.
影响因子：
158.5
作者：
Keech, Cheryl;Albert, Gary;Glenn, Gregory M.
通讯作者：
Glenn, Gregory M.
DOI：
10.1016/s0140-6736(20)32137-1
发表时间：
2020-11-14
期刊：
Lancet (London, England)
影响因子：
--
作者：
Poland GA;Ovsyannikova IG;Kennedy RB
通讯作者：
Kennedy RB
影响因子：
9.2
作者：
HogenEsch H;O'Hagan DT;Fox CB
通讯作者：
Fox CB
影响因子：
64.5
作者：
Dai, Lianpan;Zheng, Tianyi;Gao, George F.
通讯作者：
Gao, George F.