Transcriptional and post-transcriptional regulation of the GmaR antirepressor governs temperature-dependent control of flagellar motility in Listeria monocytogenes.
Transcriptional and post-transcriptional regulation of the GmaR antirepressor governs temperature-dependent control of flagellar motility in Listeria monocytogenes.
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DOI：
10.1111/j.1365-2958.2009.06874.x
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发表时间：
2009-10
影响因子：
3.6
通讯作者：
Higgins DE
中科院分区：
文献类型：
--
作者：
Kamp HD;Higgins DE
单核增生李斯特菌（Listeria monocytogenes, Lm）的鞭毛运动受限于37℃以下的温度，这是因为MogR转录抑制因子和GmaR抗抑制因子的活性相反。先前的研究表明，DegU应答调节因子和MogR都能调节GmaR的表达。在本报告中，我们进一步定义了DegU在GmaR产生和鞭毛运动中的作用。我们证明DegU受体结构域的缺失对Lm鞭毛运动没有影响。利用转录报告基因融合，我们确定gmaR在以fliN起始的操纵子内共转录。此外，fliN-gmaR启动子（pfliN-gmaR）被DegU转录激活，也受到mogr的抑制。DNA亲和纯化、凝胶迁移和足迹分析表明，DegU和MogR都直接结合fliN-gmaR启动子区DNA，并且结合位点不重叠。对ΔmogR细菌中gmaR转录本的定量分析表明，DegU对pfliN-gmaR的转录激活并不依赖于温度。然而，在ΔmogR细菌中，GmaR蛋白在37°C下检测不到，这表明温度依赖的转录后机制限制了GmaR的产生，温度低于37°C。我们的研究结果表明，Lm中的鞭毛运动受GmaR抗抑制因子的温度依赖性转录和转录后调控的控制。
Flagellar motility in Listeria monocytogenes (Lm) is restricted to temperatures below 37°C due to the opposing activities of the MogR transcriptional repressor and the GmaR anti-repressor. Previous studies have suggested that both the DegU response regulator and MogR regulate expression of GmaR. In this report, we further define the role of DegU for GmaR production and flagellar motility. We demonstrate that deletion of the receiver domain of DegU has no effect on flagellar motility in Lm. Using transcriptional reporter fusions, we determined that gmaR is co-transcribed within an operon initiating with fliN. Furthermore, the fliN-gmaR promoter (pfliN-gmaR) is transcriptionally activated by DegU and is also MogR-repressed. DNA affinity purification, gel mobility shift, and footprinting analyses revealed that both DegU and MogR directly bind fliN-gmaR promoter region DNA and that the binding sites do not overlap. Quantitative analysis of gmaR transcripts in ΔmogR bacteria indicated that transcriptional activation of pfliN-gmaR by DegU is not inherently temperature-dependent. However, GmaR protein was not detectable at 37°C in ΔmogR bacteria, indicating that a temperature-dependent, post-transcriptional mechanism limits GmaR production to temperatures below 37°C. Our findings reveal that flagellar motility in Lm is governed by both temperature-dependent transcriptional and post-transcriptional regulation of the GmaR anti-repressor.
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影响因子：
3.2
作者：
AKERLEY, BJ;MILLER, JF
通讯作者：
MILLER, JF
影响因子：
3.6
作者：
Kobayashi, Kazuo
通讯作者：
Kobayashi, Kazuo
影响因子：
3.6
作者：
Maamar, H;Dubnau, D
通讯作者：
Dubnau, D
DOI：
10.1073/pnas.87.1.41
发表时间：
1990-01-01
影响因子：
11.1
作者：
BOURRET, RB;HESS, JF;SIMON, MI
通讯作者：
SIMON, MI
影响因子：
3.2
作者：
Mauder, Norman;Williams, Tatjana;Beier, Dagmar
通讯作者：
Beier, Dagmar