Leukotriene and prostanoid pathway enzymes in bronchial biopsies of seasonal allergic asthmatics.
Leukotriene and prostanoid pathway enzymes in bronchial biopsies of seasonal allergic asthmatics.
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季节性过敏性哮喘支气管活检中的白三烯和前列腺素途径酶。
DOI：
10.1164/ajrccm.164.11.2008137
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Sampson,AP
中科院分区：
文献类型：
--
作者：
Seymour,ML;Rak,S;Aberg,D;Riise,GC;Penrose,JF;Kanaoka,Y;Austen,KF;Holgate,ST;Sampson,AP
分别由5-脂氧合酶（5-LO）和环氧合酶（考克斯）途径产生的半胱氨酰白三烯和前列腺素D2可引起支气管收缩、白细胞募集和支气管高反应性。我们采用免疫组织化学方法对12名过敏性哮喘患者在季节性暴露于桦树花粉之前和期间的支气管活检组织进行了5-LO和考克斯酶的细胞表达的表征。在花粉季节，支气管反应性（p = 0.004）和症状（p < 0.005）增加，呼气峰流量（PEF; p <0.02）降低。季节性活组织检查中，5-LO（p = 0.02）、5-LO激活蛋白（FLAP; p = 0.04）和白三烯（LT）A4水解酶（p = 0.05）的细胞免疫染色增加2倍，半胱氨酰-白三烯合成的末端酶LTC 4合酶的细胞免疫染色增加4倍（p = 0.02）。 考克斯-1、考克斯-2和PGD 2合酶的免疫染色未发生变化。LTC 4合酶染色增加是由于嗜酸性粒细胞增加（p = 0.035）和表达该酶的嗜酸性粒细胞比例增加（p = 0.047）。巨噬细胞也增加（p = 0.019），但肥大细胞和T淋巴细胞亚群不变。PEF和5-LO+细胞计数之间的负相关性将支气管粘膜内嗜酸性粒细胞和巨噬细胞中5-LO途径酶的表达增加与季节性过敏原暴露期间肺功能的恶化联系起来。
Cysteinyl-leukotrienes and prostaglandin D2generated by the 5-lipoxygenase (5-LO) and cyclooxygenase (COX) pathways, respectively, cause bronchoconstriction, leukocyte recruitment, and bronchial hyperresponsiveness in asthma. We characterized the cellular expression of 5-LO and COX enzymes using immunohistochemistry on bronchial biopsies from 12 allergic asthmatic patients before and during seasonal exposure to birch pollen. Bronchial responsiveness (p = 0.004) and symptoms (p < 0.005) increased and peak expiratory flow (PEF; p ⩽ 0.02) decreased in the pollen season. In-season biopsies had 2-fold more cells immunostaining for 5-LO (p = 0.02), 5-LO–activating protein (FLAP; p = 0.04), and leukotriene (LT)A4hydrolase (p = 0.05), and 4-fold more for the terminal enzyme for cysteinyl-leukotriene synthesis, LTC4synthase (p = 0.02). Immunostaining for COX-1, COX-2, and PGD2synthase was unchanged. Increased staining for LTC4synthase was due to increased eosinophils (p = 0.035) and an increased proportion of eosinophils expressing the enzyme (p = 0.047). Macrophages also increased (p = 0.019), but mast cells and T-lymphocyte subsets were unchanged. Inverse correlations between PEF and 5-LO+cell counts link increased expression of 5-LO pathway enzymes in eosinophils and macrophages within the bronchial mucosa to deterioration of lung function during seasonal allergen exposure.
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过敏性哮喘肺部的局部过敏原激发和支气管肺泡灌洗。
DOI：
10.1164/arrd.1987.135.2.433
发表时间：
1987
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Metzger,WJ;Zavala,D;Richerson,HB;Moseley,P;Iwamota,P;Monick,M;Sjoerdsma,K;Hunninghake,GW
通讯作者：
Hunninghake,GW
DOI：
10.1164/ajrccm/142.1.112
发表时间：
1990-07
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Sally E. Wenzel;Gary L. Larsen;Karen Johnston;Norbert F. Voelkel;Jay Y. Westcott
通讯作者：
Sally E. Wenzel;Gary L. Larsen;Karen Johnston;Norbert F. Voelkel;Jay Y. Westcott
影响因子：
64.5
作者：
Kanaoka, Y;Ago, H;Hayaishi, O
通讯作者：
Hayaishi, O
DOI：
10.1084/jem.193.2.255
发表时间：
2001-01-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hirai H;Tanaka K;Yoshie O;Ogawa K;Kenmotsu K;Takamori Y;Ichimasa M;Sugamura K;Nakamura M;Takano S;Nagata K
通讯作者：
Nagata K
c-Kit 配体介导未成熟小鼠肥大细胞中胞浆磷脂酶 A、前列腺素内过氧化物合酶-1 和造血前列腺素 D 合酶的表达增加以及 IgE 依赖性前列腺素 D 生成的增加 (*)
影响因子：
4.8
作者：
M. Murakami;Ryoji Matsumoto;Y. Urade;K. Austen;J. Arm
通讯作者：
J. Arm