A sensitive and selective detection method for thiol compounds using novel fluorescence probe.
A sensitive and selective detection method for thiol compounds using novel fluorescence probe.
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使用新型荧光探针灵敏、选择性检测硫醇化合物的方法。
DOI：
10.1016/j.aca.2014.09.004
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发表时间：
2014-11
影响因子：
6.2
通讯作者：
Chen, Hong-Yuan
中科院分区：
文献类型：
--
作者：
Li, Ying;Yu, Xiao-Dong;Xu, Jing-Juan;Chen, Hong-Yuan
本工作建立了一种基于荧光共振能量转移（FRET）的巯基化合物荧光探针检测方法。该荧光探针含有一个二硫键，可通过硫醇-二硫键交换反应选择性地与亲核硫醇盐反应。当在荧光探针溶液中加入巯基化合物时，由于巯基-二硫键交换反应和FRET的破坏，可以观察到明显的荧光恢复。该探针成功地用于二硫苏糖醇（DTT）、谷胱甘肽（GSH）和半胱氨酸（Cys）的测定。DTT、GSH和Cys的检出限分别为2.0 μM、0.6 μM和0.8 μM。并将此新方法应用于复方氨基酸注射液的分析。
In this work, a sensitive and selective detection method based on fluorescence resonance energy transfer (FRET) was developed for analyzing thiol compounds by using a novel fluorescent probe. The new fluorescent probe contains a disulfide bond which selectively reacts with nucleophilic thiolate through the thiol-disulfide exchange reaction. An obvious fluorescence recovery can be observed upon addition of the thiol compound in the fluorescent probe solution due to the thiol-disulfide exchange reaction and the destruction of FRET. This novel probe was successfully used to determine dithiothreitol (DTT), glutathione (GSH) and cysteine (Cys). The limits of detection (LOD) were 2.0 μM for DTT, 0.6 μM for GSH, and 0.8 μM for Cys. This new detection method was further investigated in the analysis of compound amino acid injection.
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影响因子：
4.2
作者：
McDermott, Geoffrey P.;Francis, Paul S.;Conlan, Xavier A.
通讯作者：
Conlan, Xavier A.
影响因子：
6.1
作者：
Zhenzhu Wu;Wenying Li;Jian Chen;Cong Yu
通讯作者：
Zhenzhu Wu;Wenying Li;Jian Chen;Cong Yu
影响因子：
7.4
作者：
Andrew M. Piggott;P. Karuso
通讯作者：
Andrew M. Piggott;P. Karuso
碳纳米点作为荧光探针，可快速、灵敏、无标记地检测复杂基质中的 Hg2 和生物硫醇
影响因子：
4.9
作者：
Zhou, Li;Lin, Youhui;Qu, Xiaogang
通讯作者：
Qu, Xiaogang
影响因子：
6.2
作者：
Bakirdere, Sezgin;Bramanti, Emilia;Mester, Zoltan
通讯作者：
Mester, Zoltan