Human papillomavirus types 6 and 11 mRNAs from genital condylomata acuminata
Human papillomavirus types 6 and 11 mRNAs from genital condylomata acuminata
复制标题
来自生殖器尖锐湿疣的人乳头瘤病毒 6 型和 11 型 mRNA
作者：
L. Chow;M. Nasseri;Steven Wolinsky;T. Broker
我们已经确定并绘制了一些RNA物种的人乳头瘤病毒6型和11型尖锐湿疣的电子显微镜R环技术。从DNA序列推导的每个早期（E）-和晚期（L）-区域开放阅读框（ORF）在一个或多个转录物中表示。此外，RNA种类，可以编码的DNA复制的调制器和转录的阻遏物，最近发现的功能，在遗传相似的牛乳头瘤病毒1型，也被检测到。一些ORF在一个或多个转录本中是5'近端的，而另一些在任何物种中都不是5'近端的，这表明可能需要内部翻译起始来获得这些后者的ORF。几乎所有转录物的5'末端都位于E区，并且在两个位点之一被聚腺苷酸化，即，在E区的末端或在L区的末端。绝大多数RNA来自基因组的E区，其中一个物种的丰度比其他物种高出约50至100倍。对于大多数RNA，5'端定位在核苷酸700附近;少数群体具有核苷酸100或1200附近的5'端。通过将我们的映射数据与几种乳头瘤病毒的基因组DNA序列以及可用的RNA结构和cDNA序列相关联，我们预测了一些mRNA剪接供体和受体位点，并表明乳头瘤病毒通过替代启动子、mRNA剪接位点和多聚腺苷酸化位点对ORF进行了复杂的使用。
We have identified and mapped a number of RNA species of human papillomavirus types 6 and 11 from condylomata acuminata by the electron microscopic R-loop technique. Each of the early (E)- and late (L)-region open reading frames (ORFs) deduced from the DNA sequences was represented in one or more transcripts. In addition, RNA species that could encode the modulator of DNA replication and the repressor of transcription, functions recently identified in the genetically similar bovine papillomavirus type 1, were also detected. Some ORFs were 5' proximal in one or more transcripts, whereas others were not 5' proximal in any species, suggesting that internal initiation of translation might be required to gain access to these latter ORFs. Virtually all transcripts had their 5' ends located in the E region and were polyadenylated at one of two sites, i.e., at the end of the E region or at the end of the L region. The great majority of the RNAs were derived from the E region of the genome, with one species approximately 50 to 100 times more abundant than the others. For most of the RNAs, the 5' end mapped near nucleotide 700; minor populations had 5' ends near nucleotide 100 or 1200. By correlating our mapping data with the genomic DNA sequences as well as available RNA structures and cDNA sequences of several papillomaviruses, we predict a number of mRNA splice donor and acceptor sites and suggest that the papillomaviruses have sophisticated usage of ORFs through alternative promoters, mRNA splice sites, and polyadenylation sites.
DOI：
10.1073/pnas.83.13.4680
发表时间：
1986-07-01
影响因子：
11.1
作者：
SMOTKIN, D;WETTSTEIN, FO
通讯作者：
WETTSTEIN, FO
肖普乳头瘤病毒在良性和恶性兔肿瘤中的转录。
影响因子：
3.7
作者：
Phelps,WC;Leary,SL;Faras,AJ
通讯作者：
Faras,AJ