Investigating fluorescent dyes in fluorescence-assisted screenings.
Investigating fluorescent dyes in fluorescence-assisted screenings.
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DOI:
10.1039/c4cc07008a
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发表时间:
2014-12-14
期刊:
影响因子:
--
通讯作者:
Lee SS
中科院分区:
文献类型:
--
作者:
Jee JE;Lim J;Hyun H;Oon J;Ong YS;Massif C;Chang YT;Choi HS;Lee SS
Screening of bead-based peptide libraries against fluorescence-labeled target proteins was found significantly influenced by the dye characteristics. Commercially available red fluorescence dyes with net negative charges adversely showed strong interactions with library beads. The introduction of zwitterionic dyes significantly reduced the unwanted interactions, which sheds light upon using the right fluorescence probe for acquisition of reliable results in various fluorescence-assisted applications.
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