Multiparametric, longitudinal optical coherence tomography imaging reveals acute injury and chronic recovery in experimental ischemic stroke.
Multiparametric, longitudinal optical coherence tomography imaging reveals acute injury and chronic recovery in experimental ischemic stroke.
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DOI：
10.1371/journal.pone.0071478
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ayata C
中科院分区：
文献类型：
--
作者：
Srinivasan VJ;Mandeville ET;Can A;Blasi F;Climov M;Daneshmand A;Lee JH;Yu E;Radhakrishnan H;Lo EH;Sakadžić S;Eikermann-Haerter K;Ayata C
脑卒中实验和转化医学的进展可以通过小鼠皮层疾病进展的高分辨率体内成像来加速。在这里，我们介绍光学显微镜的方法，监测脑损伤的进展，利用固有的光学散射特性的皮质组织。描述了一种多参数光学相干断层扫描（OCT）平台，通过薄颅骨，加强颅窗手术准备，对小鼠缺血性中风进行纵向成像。在急性期，血流动力学和细胞活力之间的时空相互作用（发病机制的关键决定因素）被成像。在急性卒中中，最终梗死的微观生物标志物，包括毛细血管不灌注、脑血流不足、细胞散射改变和脑血流自动调节受损，被量化并与组织学相关。此外，纵向显微镜显示慢性中风一周后重塑和血流恢复。本征散射特性可作为实验性脑卒中急性细胞和血管损伤及恢复的报告。多参数OCT是一个强大的体内成像平台，可以全面研究这些特性。
Progress in experimental stroke and translational medicine could be accelerated by high-resolution in vivo imaging of disease progression in the mouse cortex. Here, we introduce optical microscopic methods that monitor brain injury progression using intrinsic optical scattering properties of cortical tissue. A multi-parametric Optical Coherence Tomography (OCT) platform for longitudinal imaging of ischemic stroke in mice, through thinned-skull, reinforced cranial window surgical preparations, is described. In the acute stages, the spatiotemporal interplay between hemodynamics and cell viability, a key determinant of pathogenesis, was imaged. In acute stroke, microscopic biomarkers for eventual infarction, including capillary non-perfusion, cerebral blood flow deficiency, altered cellular scattering, and impaired autoregulation of cerebral blood flow, were quantified and correlated with histology. Additionally, longitudinal microscopy revealed remodeling and flow recovery after one week of chronic stroke. Intrinsic scattering properties serve as reporters of acute cellular and vascular injury and recovery in experimental stroke. Multi-parametric OCT represents a robust in vivo imaging platform to comprehensively investigate these properties.
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影响因子：
6.3
作者：
HEISS, WD;HUBER, M;WIENHARD, K
通讯作者：
WIENHARD, K
影响因子：
3.5
作者：
Jones, Phill B.;Shin, Hwa Kyoung;Dunn, Andrew K.
通讯作者：
Dunn, Andrew K.
影响因子：
48
作者：
Drew, Patrick J.;Shih, Andy Y.;Driscoll, Jonathan D.;Knutsen, Per Magne;Blinder, Pablo;Davalos, Dimitrios;Akassoglou, Katerina;Tsai, Philbert S.;Kleinfeld, David
通讯作者：
Kleinfeld, David
DOI：
10.1073/pnas.95.26.15741
发表时间：
1998-12-22
影响因子：
11.1
作者：
Kleinfeld, D;Mitra, PP;Denk, W
通讯作者：
Denk, W
影响因子：
5.3
作者：
Li, Ping;Murphy, Timothy H.
通讯作者：
Murphy, Timothy H.