Menstrual endometrial cells from women with endometriosis demonstrate increased adherence to peritoneal cells and increased expression of CD44 splice variants.
Menstrual endometrial cells from women with endometriosis demonstrate increased adherence to peritoneal cells and increased expression of CD44 splice variants.
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DOI：
10.1016/j.fertnstert.2008.12.012
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发表时间：
2010-04
影响因子：
6.7
通讯作者：
Schenken RS
中科院分区：
文献类型：
--
作者：
Griffith JS;Liu YG;Tekmal RR;Binkley PA;Holden AE;Schenken RS
我们先前已经证明，子宫内膜上皮(EECS)和间质细胞(ESCs)与腹膜间皮细胞(PMC)的黏附部分受ESC/EEC CD44与PMC相关的透明质酸相互作用的调节。CD44是一种跨膜糖蛋白，也是透明质酸的主要配体，它有大量的剪接变异体，可能会影响透明质酸的结合。在这里，我们评估了来自子宫内膜异位症患者的ESCs和EECs是否表现出对PMC的粘附性增加，并检查了CD44剪接变异体在这一过程中的潜在作用。体外研究。学术医学中心生育患者合并和不合并子宫内膜异位症月经期子宫内膜收集自经手术证实的子宫内膜异位症和非子宫内膜异位症患者。测定ESC/EECS与PMCs的粘附性。用斑点印迹法检测ESC/EEC CD44剪接变异体。来自子宫内膜异位症患者的ESCs和EECs显示对PMC的粘附性增加。子宫内膜异位症患者的ESCs和EECs表达的CD44剪接变异体主要为v3、v6、v7、v8、v9和v10。子宫内膜异位症患者的ESCs和EECs表达V6、V7、V8或V9的可能性较大。在位子宫内膜-PMC黏附增加和CD44剪接变异体表达可能参与了子宫内膜异位病变的组织发生。阐明控制这种表达的因素可能会导致新的子宫内膜异位症治疗方法。
We previously demonstrated that adherence of endometrial epithelial (EECs) and stromal cells (ESCs) to peritoneal mesothelial cells (PMCs) is partly regulated by ESC/EEC CD44 interactions with PMC associated hyaluronan. CD44, a transmembrane glycoprotein and major ligand for hyaluronan, has numerous splice variants which may impact hyaluronan binding. Here, we assessed whether ESCs and EECs from women with endometriosis demonstrate increased adherence to PMCs and examined CD44 splice variants’ potential role in this process. In vitro study. Academic medical Center Fertility patients with and without endometriosis Menstrual endometrium was collected from women with and without endometriosis confirmed surgically. The adherence of ESC/EECs to PMCs was measured. ESC/EEC CD44 splice variants were assessed using dot blot analysis. ESCs and EECs from women with endometriosis demonstrated increased adherence to PMCs. The predominant CD44 splice variants expressed by ESCs and EECs from women with and without endometriosis were v3, v6, v7, v8, v9, and v10. ESCs and EECs from women with endometriosis were more likely to express v6, v7, v8 or v9. Increased eutopic endometrial-PMC adherence and CD44 splice variant expression may contribute to the histogenesis of endometriotic lesions. Elucidation of factors controlling this expression may lead to novel endometriosis therapies.
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影响因子：
6
作者：
Lessan, K;Aguiar, DJ;Skubitz, APN
通讯作者：
Skubitz, APN
DOI：
10.1083/jcb.131.6.1623
发表时间：
1995-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bennett KL;Modrell B;Greenfield B;Bartolazzi A;Stamenkovic I;Peach R;Jackson DG;Spring F;Aruffo A
通讯作者：
Aruffo A
影响因子：
3.3
作者：
YAEGASHI, N;FUJITA, N;NAKAMURA, M
通讯作者：
NAKAMURA, M
DOI：
10.1016/s0889-8545(05)70302-8
发表时间：
1997-06-01
影响因子：
3.2
作者：
Eskenazi, B;Warner, ML
通讯作者：
Warner, ML
DOI：
10.1084/jem.182.2.431
发表时间：
1995-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Lesley J;English N;Perschl A;Gregoroff J;Hyman R
通讯作者：
Hyman R