Host-specific assemblages typify gut microbial communities of related insect species.
Host-specific assemblages typify gut microbial communities of related insect species.
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DOI：
10.1186/2193-1801-3-138
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Moran NA
中科院分区：
文献类型：
--
作者：
Sabree ZL;Moran NA
微生物和昆虫之间的互惠作用是普遍存在的，并且促进了宿主昆虫对各种营养生态位的利用。网翅目（螳螂、蟑螂和白蚁）表现出从杂食性到严格的木材喂养的营养作用，并与专性内共生体（Blattabacterium）和/或包括纤维素分解微生物和重氮营养微生物的多种肠道微生物组保持有益的共生。虽然在杂食性大蠊中的Blattabacterium完全能够提供必需氨基酸，但在以木材为食的网翅目中，它已经失去了许多用于其生物合成的基因（乳白蚁和隐尾目）或完全不存在（异白蚁）。在大多数严格的食木性的网翅目昆虫中明显的功能退化和Blattabacterium的缺乏表明，正在采用替代手段来获取在其饮食中有限的营养。利用16 S rRNA基因扩增子重测序技术，深入研究了近缘鳞翅目昆虫肠道群落的组成和多样性，探讨了白蚁和蟑螂进化过程中共生体亲和性发生变化的可能性。大蠊的肠道微生物组具有全功能的Blattabacterium，其表现出比乳白蚁和隐尾目动物最大的样本内操作分类单位（OTU）多样性和丰度变异性，乳白蚁和隐尾目动物的Blattabacterium具有缩小的基因组和降低的营养供应能力。异白蚁缺乏Blattabacterium和单一OTU，其与厌氧纤维素分解原生生物白蚁肠道居民样品的Bacteroidia分配的重氮营养内共生体具有95%的同一性，并且始终主导其肠道微生物组。在所有宿主属中鉴定了许多宿主特异性OTU，其中一些先前未检测到，表明通过pyrotag测序的深度采样揭示了仍待功能表征的新分类群。需要进一步的分析来揭示蟑螂和白蚁肠道微生物组中检测到的分类群以及整个群落如何有助于宿主饮食选择并影响网翅目中Blattabacterium的命运。本文的在线版本（doi：10.1186/2193-1801-3-138）包含补充材料，可供授权用户使用。
Mutualisms between microbes and insects are ubiquitous and facilitate exploitation of various trophic niches by host insects. Dictyopterans (mantids, cockroaches and termites) exhibit trophisms that range from omnivory to strict wood-feeding and maintain beneficial symbioses with the obligate endosymbiont, Blattabacterium, and/or diverse gut microbiomes that include cellulolytic and diazotrophic microbes. While Blattabacterium in omnivorous Periplaneta is fully capable of provisioning essential amino acids, in wood-feeding dictyopterans it has lost many genes for their biosynthesis (Mastotermes and Cryptocercus) or is completely absent (Heterotermes). The conspicuous functional degradation and absence of Blattabacterium in most strict wood-feeding dictyopteran insects suggest that alternative means of acquiring nutrients limited in their diet are being employed. A 16S rRNA gene amplicon resequencing approach was used to deeply sample the composition and diversity of gut communities in related dictyopteran insects to explore the possibility of shifts in symbiont allegiances during termite and cockroach evolution. The gut microbiome of Periplaneta, which has a fully functional Blattabacterium, exhibited the greatest within-sample operational taxonomic unit (OTU) diversity and abundance variability than those of Mastotermes and Cryptocercus, whose Blattabacterium have shrunken genomes and reduced nutrient provisioning capabilities. Heterotermes lacks Blattabacterium and a single OTU that was 95% identical to a Bacteroidia-assigned diazotrophic endosymbiont of an anaerobic cellulolytic protist termite gut inhabitant samples consistently dominates its gut microbiome. Many host-specific OTUs were identified in all host genera, some of which had not been previously detected, indicating that deep sampling by pyrotag sequencing has revealed new taxa that remain to be functionally characterized. Further analysis is required to uncover how consistently detected taxa in the cockroach and termite gut microbiomes, as well as the total community, contribute to host diet choice and impact the fate of Blattabacterium in dictyopterans. The online version of this article (doi:10.1186/2193-1801-3-138) contains supplementary material, which is available to authorized users.
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影响因子：
56.9
作者：
BENEMANN, JR
通讯作者：
BENEMANN, JR
影响因子：
2.2
作者：
Carpenter, Kevin J.;Horak, Ales;Keeling, Patrick J.
通讯作者：
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影响因子：
56.9
作者：
Hongoh, Yuichi;Sharma, Vineet K.;Ohkuma, Moriya
通讯作者：
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影响因子：
2.7
作者：
CHAO, A;MA, MC;YANG, MCK
通讯作者：
YANG, MCK
影响因子：
3
作者：
Camacho C;Coulouris G;Avagyan V;Ma N;Papadopoulos J;Bealer K;Madden TL
通讯作者：
Madden TL