Transactivation by the adenovirus E1A gene.-猫眼课题宝

腺病毒 E1A 基因的反式激活。

DOI：

发表时间：

1988

期刊：

Biochemistry and cell biology = Biochimie et biologie cellulaire

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

J. Nevins;P. Raychaudhuri;A. Yee;R. J. Rooney;I. Kovesdi;R. Reichel

腺病毒E1a基因的289aa产物介导了一组早期病毒基因以及几个细胞基因的转录激活。E1a蛋白不是一种DNA结合蛋白，而是间接地实现激活。病毒基因的激活过程涉及到细胞转录因子的使用，在至少一个案例中，体内试验证明了E1A基因产物能刺激稳定的启动子复合体的形成。对核提取液中转录因子的分析已经确定了一种被称为E2F的细胞因子，它对病毒E2启动子具有特异性。由于E1a的作用，这一因子的浓度增加。这种DNA结合活性的增加不需要蛋白质合成，因此表明E1A介导了对先前存在的因子的修饰。E2F因子已纯化至均一，是一种分子量为54,000的多肽。对另一种病毒启动子E4启动子的分析发现了一种与转录关键序列相互作用的蛋白质。这种被称为E4F的因子也随着E1a产物的增加而增加。E4F因子也被提纯到均一状态，分子量为50,000。因此，E1a基因产物对转录的协调控制涉及到多个启动子特异性因子的激活。

The 289aa product of the adenovirus E1A gene mediates the transcriptional activation of the set of early viral genes as well as several cellular genes. The E1A protein is not a DNA binding protein but, rather, acts indirectly to achieve the activation. The process of viral gene activation involves the use of cellular transcription factors, and in at least one case, in vivo assays have demonstrated a stimulation of stable promoter complex formation as a function of the E1A gene product. Analysis of transcription factors in nuclear extracts has identified a cellular factor, termed E2F, with specificity for the viral E2 promoter. The concentration of this factor increases as a result of the action of E1A. This increase in DNA binding activity does not require protein synthesis, thus indicating an E1A-mediated modification of a pre-existing factor. The E2F factor has been purified to homogeneity and is a polypeptide of 54,000 molecular weight. Analysis of an additional viral promoter, the E4 promoter, has identified a protein that interacts with sequences critical for transcription. This factor, termed E4F, is also increased as a function of the E1A product. The E4F factor has also been purified to homogeneity and has a molecular weight of 50,000. Therefore, the coordinate control of transcription by the E1A gene product involves the activation of multiple promoter specific factors.