The molecular and cellular signatures of the mouse eminentia thalami support its role as a signalling centre in the developing forebrain.
The molecular and cellular signatures of the mouse eminentia thalami support its role as a signalling centre in the developing forebrain.
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DOI：
10.1007/s00429-015-1127-3
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发表时间：
2016-09
影响因子：
3.1
通讯作者：
Fotaki V
中科院分区：
文献类型：
--
作者：
Adutwum-Ofosu KK;Magnani D;Theil T;Price DJ;Fotaki V
哺乳动物丘脑隆起（eminentia thalami，EmT）是一种功能未知的胚胎前脑结构。在这里，我们研究了小鼠EmT的分子和细胞特性。我们首先研究了信号分子的mRNA表达，发现EmT是一种富含分泌因子表达的结构，其中Wnt被最大量地检测到。然后，我们研究了异位移植时，EmT组织是否可以诱导细胞命运的变化。为此，我们将来自tau-GFP小鼠的EmT组织移植到野生型宿主的端脑腹侧，这是一个端脑区域，在该区域Wnt信号传导通常不活跃，但我们在培养实验中显示其能够对Wnt做出反应。我们观察到，EmT能够诱导邻近腹侧端脑细胞中Lef 1的异位表达，Lef 1是Wnt/β-catenin通路的转录激活因子和靶基因。这些Lef 1阳性; GFP阴性细胞表达端脑标志物Foxg 1，但不表达Ascl 1，Ascl 1通常由腹侧端脑细胞表达。这些结果表明，EmT有能力激活腹侧端脑中的Wnt/β-catenin信号传导并抑制腹侧端脑基因表达。总之，我们的数据支持的作用，作为一个信号中心的EmT在小鼠前脑的发展。本文的在线版本（doi：10.1007/s 00429 -015-1127-3）包含补充材料，可供授权用户使用。
The mammalian eminentia thalami (EmT) (or thalamic eminence) is an embryonic forebrain structure of unknown function. Here, we examined the molecular and cellular properties of the mouse EmT. We first studied mRNA expression of signalling molecules and found that the EmT is a structure, rich in expression of secreted factors, with Wnts being the most abundantly detected. We then examined whether EmT tissue could induce cell fate changes when grafted ectopically. For this, we transplanted EmT tissue from a tau-GFP mouse to the ventral telencephalon of a wild type host, a telencephalic region where Wnt signalling is not normally active but which we showed in culture experiments is competent to respond to Wnts. We observed that the EmT was able to induce in adjacent ventral telencephalic cells ectopic expression of Lef1, a transcriptional activator and a target gene of the Wnt/β-catenin pathway. These Lef1-positive;GFP-negative cells expressed the telencephalic marker Foxg1 but not Ascl1, which is normally expressed by ventral telencephalic cells. These results suggest that the EmT has the capacity to activate Wnt/β-catenin signalling in the ventral telencephalon and to suppress ventral telencephalic gene expression. Altogether, our data support a role of the EmT as a signalling centre in the developing mouse forebrain. The online version of this article (doi:10.1007/s00429-015-1127-3) contains supplementary material, which is available to authorized users.
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影响因子：
5.3
作者：
Fotaki, Vassiliki;Yu, Tian;Zaki, Paulette A;Mason, John O;Price, David J
通讯作者：
Price, David J
影响因子：
5.3
作者：
Hsu, SC;Galceran, J;Grosschedl, R
通讯作者：
Grosschedl, R
影响因子：
56.9
作者：
Mangale, Vishakha S.;Hirokawa, Karla E.;Satyaki, Prasad R. V.;Gokulchandran, Nandini;Chikbire, Satyadeep;Subramanian, Lakshmi;Shetty, Ashwin S.;Martynoga, Ben;Paul, Jolly;Mai, Mark V.;Li, Yuqing;Flanagan, Lisa A.;Tole, Shubha;Monuki, Edwin S.
通讯作者：
Monuki, Edwin S.
影响因子：
2.7
作者：
Fotaki, Vassiliki;Smith, Rowena;Pratt, Thomas;Price, David J.
通讯作者：
Price, David J.
影响因子：
10.5
作者：
LO, LC;JOHNSON, JE;ANDERSON, DJ
通讯作者：
ANDERSON, DJ