Targeted proteomics: Current status and future perspectives for quantification of food allergens.
Targeted proteomics: Current status and future perspectives for quantification of food allergens.
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DOI：
10.1016/j.jprot.2016.04.018
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发表时间：
2016-06-30
影响因子：
3.3
通讯作者：
Salomon AR
中科院分区：
文献类型：
--
作者：
Ahsan N;Rao RSP;Gruppuso PA;Ramratnam B;Salomon AR
新鲜食品和加工食品中的过敏原水平可以动态变化。由于不同来源的食物会引起不同类型的过敏反应，食品行业和监管机构迫切需要可靠的检测和绝对定量方法来检测复杂食品中的过敏原，以有效保护食物过敏人群。靶向蛋白质组学技术的最新进展，即多反应监测（MRM）质谱（MS）与同位素标记内标（也称为AQUA肽）相结合，可以以多重方式在10 ppb水平上对食物过敏原进行绝对定量。然而，开发成功的AQUA-MRM分析依赖于许多MS前和MS后标准。在这篇综述中，我们简要地描述了植物和动物为基础的食物中过敏原水平的潜在变化，需要开发一种高通量多路过敏原定量方法，以成功地进行AQUA-MRM分析。我们还提出了一些未来的策略，可以提供更好的食物过敏管理。
Allergen levels in fresh and processed foods can vary dynamically. As different sources of foods can cause different types of allergic reactions, the food industry and regulatory bodies urgently require reliable detection and absolute quantitation methods for allergen detection in complex food products to effectively safeguard the food-allergic population. Recent advances of targeted proteomic technologies namely multiple-reaction monitoring (MRM) mass spectrometry (MS) coupled with isotope-labeled internal standard, also known as AQUA peptides offers absolute quantitation of food allergens even at 10 ppb level in a multiplex fashion. However, development of successful AQUA-MRM assay relies on a number of pre and post MS criteria. In this review, we briefly describe how allergen levels could potentially change in plant and animal based foods, necessitating the development of a high throughput multiplexed allergen quantification methodology for successful AQUA-MRM assay. We also propose some future strategies that could provide better management of food allergy.
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