Circulating endothelial progenitor cells decrease in infants with bronchopulmonary dysplasia and increase after inhaled nitric oxide.
Circulating endothelial progenitor cells decrease in infants with bronchopulmonary dysplasia and increase after inhaled nitric oxide.
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患有支气管肺发育不良的婴儿的循环内皮祖细胞减少，吸入一氧化氮后增加。
DOI：
10.1371/journal.pone.0079060
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Qian L
中科院分区：
文献类型：
--
作者：
Qi Y;Jiang Q;Chen C;Cao Y;Qian L
内皮祖细胞（EPCs）的损伤已被证明有助于支气管肺发育不良（BPD）的发展。本研究旨在探讨出生后内皮祖细胞（EPC）的变化与BPD发生发展的关系，以及吸入一氧化氮（iNO）对EPCs的影响。研究了60例胎龄小于32周、出生体重小于1500 g的婴儿。在7 - 21天之间接受机械通气或CPAP至少2天的婴儿中给予NO。采用流式细胞仪检测出生时、7、21、28日龄和36周龄时、iNO治疗前后的EPC水平。采用免疫组化法测定血浆中血管内皮生长因子（VEGF）、基质细胞衍生因子-1和粒细胞-巨噬细胞集落刺激因子的浓度。25例新生儿发生BPD，35例存活且未发生BPD。与未发生BPD的婴儿相比，后来发生BPD的婴儿在第7天和第21天的EPC水平降低。从出生到21天，BPD婴儿与非BPD婴儿相比，VEGF浓度持续较低。PMA第28、36周两组间无差异。在后来发展为BPD的婴儿中，iNO使KDR+ CD 133+和CD 34 +KDR+ CD 133 + EPC数量沿着血浆VEGF水平升高。BPD患儿7日龄时EPC水平降低，iNO沿着VEGF水平升高而使EPC数量增加。BPD患儿EPCs减少的机制及iNO治疗在BPD预防中的作用有待进一步研究。
Impairment of endothelial progenitor cells (EPCs) has been shown to contribute to the development of bronchopulmonary dysplasia (BPD). In the current study, the relationship between EPC changes of after birth and the development of BPD was investigated, and the effects of inhaled nitric oxide (iNO) on EPCs were evaluated. Sixty infants with a gestational age of less than 32 weeks and a birth weight of less than 1500 g were studied. NO was administered to infants who were receiving mechanical ventilation or CPAP for at least 2 days between the ages of 7 and 21 days. EPC level was determined by flow cytometry at birth, 7, 21 and 28 days of age and 36 weeks’ postmenstrual age (PMA), before and after the iNO treatment. Plasma concentrations of vascular endothelial growth factor (VEGF), stromal cell-derived factor-1 and granulocyte-macrophage colony-stimulating factor were determined via immunochemical assay. Twenty-five neonates developed BPD, 35 neonates survived and did not develop BPD. EPC level was decreased on day 7 and 21 in infants who later developed BPD compared with infants that did not develop BPD. From birth to 21 days of age, BPD infants had a persistently lower VEGF concentration compared with non-BPD infants. No difference was found between the two groups at day 28 or 36 weeks PMA. In infants that later developed BPD, iNO raised the KDR+CD133+ and CD34+KDR+CD133+ EPC numbers along with increasing the level of plasma VEGF. EPC level was reduced at 7 days of age in infants with BPD, and iNO increased the EPC number along with increasing the level of VEGF. Further studies are needed to elucidate the mechanism leading to the decrease of EPCs in infants with BPD and to investigate the role of iNO treatment in the prevention of BPD.
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DOI：
10.1165/ajrcmb.20.1.3251
发表时间：
1999-01-01
影响因子：
6.4
作者：
Acarregui, MJ;Penisten, ST;Snyder, JM
通讯作者：
Snyder, JM
DOI：
10.1164/rccm.200812-1949oc
发表时间：
2009-09-15
影响因子：
24.7
作者：
Borghesi, Alessandro;Massa, Margherita;Stronati, Mauro
通讯作者：
Stronati, Mauro
影响因子：
158.5
作者：
Ballard, Roberta A.;Truog, William E.;Coburn, Christine E.
通讯作者：
Coburn, Christine E.
DOI：
10.1073/pnas.041359198
发表时间：
2001-02-27
影响因子：
11.1
作者：
Fukumura, D;Gohongi, T;Jain, RK
通讯作者：
Jain, RK
影响因子：
82.9
作者：
Aicher, A;Heeschen, C;Dimmeler, S
通讯作者：
Dimmeler, S