In vitro culturing of porcine tracheal mucosa as an ideal model for investigating the influence of drugs on human respiratory mucosa.
In vitro culturing of porcine tracheal mucosa as an ideal model for investigating the influence of drugs on human respiratory mucosa.
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DOI：
10.1007/s00405-008-0661-5
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发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
Mickenhagen A
中科院分区：
文献类型：
--
作者：
Stennert E;Siefer O;Zheng M;Walger M;Mickenhagen A
以往的研究表明，不同哺乳动物的新鲜粘膜可以作为体外培养的原料，纤毛的分化是粘膜纤毛系统功能的最重要的形态结构。越来越多的法律限制切除人体组织，以及不断变化的外科技术，导致缺乏可供培养的新鲜人体粘膜。到目前为止，大多数被用作捐赠者的动物在基因上与人类不是很亲近，必须为此类研究牺牲所有动物。因此，我们建立了一种改良的从猪的气管培养粘膜细胞的系统，这种细胞可以作为屠宰后的常规副产品使用。关于纤毛形成的可能性，可以看出，猪和人的粘膜细胞在贴壁之前的细胞增殖速度、细胞团的结合形成和纤毛的增殖是相似的。此外，可以证明，猪纤毛搏动频率为7.57±111.39赫兹，与人粘膜细胞搏动频率7.3±111.4赫兹相当，并且在360分钟的调查时间内，该搏动频率是绝对恒定的。为了证明猪和人的纤毛对不同药物的反应是否具有可比性，我们首先测试了已知对人类细胞有毒的苯扎氯铵，然后测试了在之前对人类黏膜的研究中发现无毒的那苯唑啉。结果清楚地表明，猪纤毛细胞对这些物质的功能和形态反应与我们在体外培养的人粘膜中发现的反应相似。
It has been previously shown that fresh mucosa from different mammals could serve as raw material for in vitro culturing with the differentiation of cilia, which are the most important morphological structures for the function of the mucociliary system. Increasing legal restrictions on the removal of human tissue and changing surgical techniques have led to a lack of fresh human mucosa for culturing. Most of the animals that have been used as donors up to now are genetically not very close to human beings and must all be sacrificed for such studies. We, therefore, established a modified system of culturing mucosa cells from the trachea of pigs, which is available as a regular by-product after slaughtering. With respect to the possibility of developing “beating” cilia, it could be shown that the speed of cell proliferation until adhesion to the coated culture dishes, the formation of conjunctions of cell clusters and the proliferation of cilia were comparable for porcine and human mucosa. Moreover, it could be demonstrated that the porcine cilia beat frequency of 7.57 ± 1.39 Hz was comparable to the human mucosa cells beat frequency of 7.3 ± 1.4 Hz and that this beat frequency was absolutely constant over the investigation time of 360 min. In order to prove whether the reaction to different drugs is comparable between the porcine and human cilia, we initially tested benzalkonium chloride, which is known to be toxic for human cells, followed by naphazoline, which we found in previous studies on human mucosa to be non-toxic. The results clearly showed that the functional and morphological reactions of the porcine ciliated cells to these substances were similar to the reaction we found in the in vitro cultured human mucosa.
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DOI：
10.1111/j.1540-8159.1984.tb04885.x
发表时间：
1984-01-01
影响因子：
1.8
作者：
BOWMAN, TA;HUGHES, HC
通讯作者：
HUGHES, HC
影响因子：
1
作者：
Mickenhagen, A.;Siefer, O.;Stennert, E.
通讯作者：
Stennert, E.
影响因子：
2.6
作者：
Neugebauer, P;Endepols, H;Walger, M
通讯作者：
Walger, M
影响因子：
6
作者：
Wyatt, TA;Forgèt, MA;Sisson, JH
通讯作者：
Sisson, JH
影响因子：
5.5
作者：
Salathe, M;Bookman, RJ
通讯作者：
Bookman, RJ