Enhanced endothelial cytopathogenicity induced by a cytomegalovirus strain propagated in endothelial cells
Enhanced endothelial cytopathogenicity induced by a cytomegalovirus strain propagated in endothelial cells
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在内皮细胞中繁殖的巨细胞病毒株诱导的内皮细胞致病性增强
DOI：
10.1002/jmv.1890280405
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发表时间：
1989
影响因子：
12.7
通讯作者：
W. Roberts
中科院分区：
文献类型：
--
作者：
W. James Waldman;J. Sneddon;R. Stephens;W. Roberts
巨细胞病毒(CMV)感染是免疫抑制患者(如获得性免疫缺陷综合征[AIDS]患者和器官移植患者)主要并发症的来源，内皮细胞的参与与巨细胞病毒感染有关。传统上，CMV是在成纤维细胞中培养的；然而，在这些细胞中繁殖可能会改变病毒的特征。在建立巨细胞病毒/内皮细胞相互作用的体外模型系统中，我们通过将严重感染的培养物与新鲜的人脐静脉内皮细胞(HUVE)进行连续共培养，在人脐静脉内皮细胞(HUVE)中繁殖了CMV VHL-1临床分离株，并比较了其与成纤维细胞培养的CMV AD169株的感染特性。使用生物素标记的DNA探针的原位杂交，以及CMV特异性抗原的免疫荧光染色，证实了接种了任一病毒株的HUVE细胞受到了感染。VHL感染HUVE伴随着AD169感染细胞没有观察到的戏剧性细胞病理学。培养上清液的空斑分析表明，与同等接种的成纤维细胞相比，VHL感染的HUVE产生了更多的病毒。相反，在接种的成纤维细胞中，AD169的产量高于HUVE。这些研究表明，培养的内皮细胞适合作为CMV增殖的底物，并表明这样繁殖的病毒株可能提供人类疾病中CMV/内皮细胞相互作用的准确模型。
Endothelial involvement has been implicated in cytomegalovirus (CMV) infection, a source of major complications in immunosuppressed individuals (e.g., those with acquired immune deficiency syndrome [AIDS] and organ transplants). Traditionally, CMV has been grown in fibroblasts; however, propagation in these cells may alter characteristics of the virus. In developing an in vitro model system of CMV/endothelial cell interaction, we have addressed this issue by propagating a clinical isolate, CMV VHL 1, in human umbilical vein endothelial (HUVE) cells by serial cocultivation of heavily infected cultures with fresh HUVE monolayers and have compared its infectious properties with those of the fibroblast‐raised strain, CMV AD169. In situ hybridization using a biotinylated DNA probe, as well as immunofluorescent staining for CMV specific antigen, has confirmed infection of HUVE cells inoculated with either strain of the virus. Infection of HUVE by VHL was accompanied by dramatic cytopathology not observed in AD169‐infected cells. Plaque assay of culture supernatants revealed greater virus production in VHL‐infected HUVE as compared with equivalently inoculated fibroblasts. In contrast, AD169 production in inoculated fibroblasts exceeded that in HUVE. These studies demonstrate the suitability of cultured endothelial cells as a substrate for CMV propagation and suggest that a strain of virus thus propagated may offer an accurate model of CMV/endothelial cell interaction in human disease.
心脏移植受者的特异性细胞介导的免疫和疱疹病毒感染。
DOI：
10.1016/0002-9343(82)90410-7
发表时间：
1982
期刊：
The American journal of medicine
影响因子：
--
作者：
Pollard,RB;Arvin,AM;Gamberg,P;Rand,KH;Gallagher,JG;Merigan,TC
通讯作者：
Merigan,TC
影响因子：
3.3
作者：
MYERSON, D;HACKMAN, RC;MCDOUGALL, JK
通讯作者：
MCDOUGALL, JK
骨髓移植后弥漫性肠溃疡：13 例患者的临床病理学研究。
影响因子：
3.3
作者：
Spencer,GD;Shulman,HM;Myerson,D;Thomas,ED;McDonald,GB
通讯作者：
McDonald,GB